Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

C3 receptors on lymphoid-cells - isolation of active membrane fragments and solubilization of receptor complexes

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Dierich, M. P.
  • Reisfeld, Ralph

publication date

  • 1975

journal

  • Journal of Immunology  Journal

abstract

  • Complement receptor activity for cell bound C3b and C3d was detected on plasma membrane fragments prepared by nitrogen cavitation from cultured human lymphoid cells. The activity of the membrane fragments reflected the activity of the whole cells in that cells which did not form rosettes (P3J and RPMI 4098) resulted in inactive membranes and cells with high rosette formation (NC37 and Raji) yielded highly active membrane fragments. Two test systems were devised to detect these receptor activities, namely a rosette inhibition and a hemagglutination assay. Solubilization of C3 receptors was accomplished by extraction of active plasma membrane fragments with 2 MKBr. Dissociation and reassociation experiments suggest C3b and C3d receptors to be highly complex molecular structures. It appears that these complement receptors on plasma membranes rely on both protein and lipid moieties for the expression of their activity.

subject areas

  • Binding Sites
  • Burkitt Lymphoma
  • Butanols
  • Cell Fractionation
  • Cell Line
  • Cell Membrane
  • Cells, Cultured
  • Complement C3
  • Complement System Proteins
  • Deoxycholic Acid
  • Electrophoresis, Polyacrylamide Gel
  • Erythrocytes
  • Ethanol
  • HEPES
  • Hemagglutination Tests
  • Humans
  • Immune Adherence Reaction
  • Leukemia, Myeloid
  • Lipids
  • Lymphocytes
  • Neoplasm Proteins
  • Polyethylene Glycols
  • Sodium Dodecyl Sulfate
  • Sucrose
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 1055171
scroll to property group menus

Additional Document Info

start page

  • 1676

end page

  • 1682

volume

  • 114

issue

  • 6

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support