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In vivo identification of glycolipid antigen-specific T cells using fluorescent CD1d tetramers

Academic Article
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Overview

authors

  • Benlagha, K.
  • Weiss, A.
  • Beavis, A.
  • Teyton, Luc
  • Bendelac, A.

publication date

  • June 2000

journal

  • Journal of Experimental Medicine  Journal

abstract

  • The CD1 family of major histocompatibility complex (MHC)-like molecules specializes in presenting lipid and glycolipid antigens to alpha/beta T lymphocytes, but little is known about the size of the CD1-restricted T cell population or the frequency of T lymphocytes specific for a given glycolipid antigen. Here, we report the generation and use of mouse CD1d1-glycolipid tetramers to visualize CD1d-restricted T cells. In contrast with previous BIAcore-based estimates of very short half-lives for CD1d-glycolipid complexes, we found that the dissociation rate of several different CD1d-glycolipid complexes was very slow. Fluorescent tetramers of mouse CD1d1 complexed with alpha-galactosylceramide (alphaGalCer), the antigen recognized by mouse Valpha14-Jalpha281/Vbeta8 and human Valpha24-JalphaQ/Vbeta11 natural killer T (NKT) cell T cell receptors (TCRs), allowed us for the first time to accurately describe, based on TCR specificity, the entire population of NKT cells in vivo and to identify a previously unrecognized population of NK1.1-negative "NKT" cells, which expressed a different pattern of integrins. In contrast, natural killer (NK) cells failed to bind the tetramers either empty or loaded with alphaGalCer, suggesting the absence of a CD1d-specific, antigen-nonspecific NK receptor. Mouse CD1d1-alphaGalCer tetramers also stained human NKT cells, indicating that they will be useful for probing a range of mouse and human conditions such as insulin-dependent diabetes mellitus, tumor rejection, and infectious diseases where NKT cells play an important role.

subject areas

  • Animals
  • Antigens
  • Antigens, CD1
  • Antigens, CD1d
  • Cells, Cultured
  • Female
  • Fluorescent Dyes
  • Galactosylceramides
  • Humans
  • Killer Cells, Natural
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Receptors, Antigen, T-Cell, alpha-beta
  • Staining and Labeling
  • T-Lymphocytes
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Research

keywords

  • T cell development
  • T cell receptor
  • V alpha 14 NKT cell
  • antigen presentation
  • natural killer cell
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Identity

PubMed Central ID

  • PMC2213523

International Standard Serial Number (ISSN)

  • 0022-1007

PubMed ID

  • 10839805
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Additional Document Info

start page

  • 1895

end page

  • 1903

volume

  • 191

issue

  • 11

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