Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Subtractive cloning of complementary dnas and analysis of messenger-rnas with regional heterogeneous distributions in primate cortex

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Travis, G. H.
  • Naus, C. G.
  • Morrison, J. H.
  • Bloom, Floyd
  • Sutcliffe, J. Gregor

publication date

  • July 1987

journal

  • Neuropharmacology  Journal

abstract

  • Morphological heterogeneity of individual neurons in the mammalian brain must ultimately result from differences between cells in their profiles of gene expression. However, the degree to which neurons located in different regions of the brain express different sets of genes is not known. Using differential cDNA cloning procedures, including subtractive hybridization and differential colony screening, a quantitative analysis of RNAs with heterogeneous distributions in the telencephalon of the rat and Old World monkey has been performed. The results suggest that no species of RNA exist with a distribution specific to the hippocampus or neocortex of the rat with an abundance greater than 0.05%. Additionally, the results with an abundance of 0.05% or greater which is present in neocortex of the monkey but absent from the cerebellum suggest that only one species of mRNA exists. A Northern blot analysis of cDNA clones representing mRNAs present in the neocortex but absent from the cerebellum of the monkey is presented, establishing the effectiveness of subtractive hybridization between different regions of the brain for cDNA cloning. Also presented is in situ hybridization analysis of the cellular distributions in primate neocortex of two mRNAs. One of these RNAs, detected by clone 1B4 is present in cortical laminae V and VI, and exhibits a high degree of heterogeneity in the overall density of labelled cells.

subject areas

  • Animals
  • Cercopithecidae
  • Cerebral Cortex
  • Cloning, Molecular
  • DNA
  • DNA, Single-Stranded
  • Nucleic Acid Hybridization
  • Poly A
  • RNA
  • RNA, Messenger
  • Rats
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0028-3908

Digital Object Identifier (DOI)

  • 10.1016/0028-3908(87)90061-x

PubMed ID

  • 2443867
scroll to property group menus

Additional Document Info

start page

  • 845

end page

  • 854

volume

  • 26

issue

  • 7B

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support