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Cofilin activity downstream of Pak1 regulates cell protrusion efficiency by organizing lamellipodium and lamella actin networks

Academic Article
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Overview

authors

  • Delorme, V.
  • Machacek, M.
  • DerMardirossian, Celine
  • Anderson, K. L.
  • Wittmann, T.
  • Hanein, D.
  • Waterman-Storer, C.
  • Danuser, G.
  • Bokoch, G. M.

publication date

  • November 2007

journal

  • Developmental Cell  Journal

abstract

  • Protrusion of the leading edge of migrating epithelial cells requires precise regulation of two actin filament (F-actin) networks, the lamellipodium and the lamella. Cofilin is a downstream target of Rho GTPase signaling that promotes F-actin cycling through its F-actin-nucleating, -severing, and -depolymerizing activity. However, its function in modulating lamellipodium and lamella dynamics, and the implications of these dynamics for protrusion efficiency, has been unclear. Using quantitative fluorescent speckle microscopy, immunofluorescence, and electron microscopy, we establish that the Rac1/Pak1/LIMK1 signaling pathway controls cofilin activity within the lamellipodium. Enhancement of cofilin activity accelerates F-actin turnover and retrograde flow, resulting in widening of the lamellipodium. This is accompanied by increased spatial overlap of the lamellipodium and lamella networks and reduced cell-edge protrusion efficiency. We propose that cofilin functions as a regulator of cell protrusion by modulating the spatial interaction of the lamellipodium and lamella in response to upstream signals.

subject areas

  • Actin Depolymerizing Factors
  • Actins
  • Cell Line
  • Cell Movement
  • Epithelial Cells
  • Fluorescent Antibody Technique
  • Humans
  • Lim Kinases
  • Microfilament Proteins
  • Pseudopodia
  • Signal Transduction
  • p21-Activated Kinases
  • rac1 GTP-Binding Protein
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Identity

PubMed Central ID

  • PMC2170459

International Standard Serial Number (ISSN)

  • 1534-5807

Digital Object Identifier (DOI)

  • 10.1016/j.devcel.2007.08.011

PubMed ID

  • 17981134
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Additional Document Info

start page

  • 646

end page

  • 662

volume

  • 13

issue

  • 5

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