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Positive signal transduction via surface cd4 molecules does not need coexpression of the cd3/tcr complex

Academic Article
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Overview

authors

  • Carrel, S.
  • Salvi, S.
  • Gallay, Philippe
  • Rapin, C.
  • Sekaly, R. P.

publication date

  • 1991

journal

  • Research in Immunology  Journal

abstract

  • We previously reported that the human CD4 molecule is capable of transducing a positive signal when activated by an anti-CD4 mAb B66. This antibody, in contrast to many other anti-CD4 mAb, induced IL2 production and proliferation of resting CD4+ peripheral blood T lymphocytes in the absence of any other signal. We further reported that anti-CD4 mAb B66 was able to induce IL2 production in murine T-cell hybridoma cells transfected with full-length human CD4 cDNA. In the present study, we extend these findings by demonstrating that anti-CD4 mAb B66 was able to induce Ca2+ mobilization and IL2 production in a CD3/TcR- variant 31-13, of the CD3/TcR+ Jurkat cell line. We further showed that anti-CD4 mAb B66 was able to activate CD4+ cells from the promonocytic cell line U937. In these cells, mAb B66 induced Ca2+ mobilization when cross-linked with a second antibody and, in addition, the production of large quantities of IL1 beta was measured. In essence, our findings provide direct evidence that cross-linking of CD4 may cause T-cell activation in the absence of the coexpression of the CD3/TcR molecular complex and that, in addition, CD4 might transduce a positive signal in CD4+ cells of the myeloid lineage.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antigens, CD3
  • Antigens, CD4
  • Antigens, Differentiation, T-Lymphocyte
  • Burkitt Lymphoma
  • CD4-Positive T-Lymphocytes
  • Calcium
  • DNA
  • Humans
  • Interleukin-1
  • Interleukin-2
  • Lymphocyte Activation
  • Lymphoma, Large B-Cell, Diffuse
  • Mice
  • Monocytes
  • Receptors, Antigen, T-Cell
  • Recombinant Proteins
  • Signal Transduction
  • Tumor Cells, Cultured
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Research

keywords

  • LYMPHOCYTE-T, TCR, CD4, HIV
  • MAB, IL1, IL2
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Identity

International Standard Serial Number (ISSN)

  • 0923-2494

Digital Object Identifier (DOI)

  • 10.1016/0923-2494(91)90017-d

PubMed ID

  • 1678202
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Additional Document Info

start page

  • 97

end page

  • 108

volume

  • 142

issue

  • 2

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