Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Ligand-induced and nonfusogenic dissolution of a viral membrane

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Law, Mansun
  • Carter, G. C.
  • Roberts, K. L.
  • Hollinshead, M.
  • Smith, G. L.

publication date

  • April 2006

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Hitherto, all enveloped viruses were thought to shed their lipid membrane during entry into cells by membrane fusion. The extracellular form of Vaccinia virus has two lipid envelopes surrounding the virus core, and consequently a single fusion event will not deliver a naked core into the cell. Here we report a previously underscribed mechanism in which the outer viral membrane is disrupted by a ligand-induced nonfusogenic reaction, followed by the fusion of the inner viral membrane with the plasma membrane and penetration of the virus core into the cytoplasm. The dissolution of the outer envelope depends on interactions with cellular polyanionic molecules and requires the virus glycoproteins A34 and B5. This discovery represents a remarkable example of how viruses manipulate biological membranes, solves the topological problem of how a double-enveloped virus enters cells, reveals a new effect of polyanions on viruses, and provides a therapeutic approach for treatment of poxvirus infections, such as smallpox.

subject areas

  • Ligands
  • Membrane Fusion
  • Microscopy, Electron
  • Myxoma virus
  • Vaccinia virus
  • Viral Envelope Proteins
  • Viral Fusion Proteins
  • Viral Plaque Assay
scroll to property group menus

Research

keywords

  • Vaccinia virus
  • antiviral therapy
  • extracellular enveloped virus
  • membrane dissolution
  • virus entry
scroll to property group menus

Identity

PubMed Central ID

  • PMC1424662

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0601025103

PubMed ID

  • 16585508
scroll to property group menus

Additional Document Info

start page

  • 5989

end page

  • 5994

volume

  • 103

issue

  • 15

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support