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Induction in vitro of a primary human antiviral cytotoxic T cell response

Academic Article
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Overview

authors

  • Cerny, A.
  • Fowler, P.
  • Brothers, M. A.
  • Houghton, M.
  • Schlicht, H. J.
  • Chisari, Francis

publication date

  • February 1995

journal

  • European Journal of Immunology  Journal

abstract

  • We report herein the successful priming of human anti-viral cytotoxic T cells (CTL) in vitro using two induction strategies based on the stimulation of peripheral blood mononuclear cells isolated from uninfected donors with synthetic viral peptides. The peptides used contain HLA-A2 binding motifs and have been identified as HLA-A2-restricted CTL epitopes in patients infected by the hepatitis B and C viruses. One approach uses repetitive long-term stimulation and the other uses bulk cultures containing large numbers of naive peripheral blood mononuclear cells. Both approaches successfully induce HLA-A2-restricted CTL specific for several viral epitopes. Some CTL recognize endogenously synthesized antigen on target cells infected with recombinant vaccinia virus expressing the corresponding viral proteins. This simple technique permits easy analysis of the primary human CTL repertoire, and may be exploitable for production of specific CTL effector cells for adoptive immunotherapy and dissection of the cellular and molecular requirements for priming of naive human CTL.

subject areas

  • Amino Acid Sequence
  • Antigens, Viral
  • Female
  • HLA-A2 Antigen
  • Hepacivirus
  • Hepatitis B virus
  • Humans
  • Male
  • Molecular Sequence Data
  • Peptide Fragments
  • T-Lymphocytes, Cytotoxic
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Research

keywords

  • CYTOTOXIC T LYMPHOCYTES
  • HEPATITIS B VIRUS
  • HEPATITIS C VIRUS
  • HLA-A2.1
  • T CELL EPITOPE
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Identity

International Standard Serial Number (ISSN)

  • 0014-2980

Digital Object Identifier (DOI)

  • 10.1002/eji.1830250248

PubMed ID

  • 7875224
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Additional Document Info

start page

  • 627

end page

  • 630

volume

  • 25

issue

  • 2

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