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Nuclear autoantigen p330d/CENP-F: a marker for cell proliferation in human malignancies

Academic Article
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Overview

authors

  • Landberg, G.
  • Erlanson, M.
  • Roos, G.
  • Tan, Eng
  • Casiano, C. A.

publication date

  • September 1996

journal

  • Cytometry  Journal

abstract

  • p330d, also known as CENP-F, is a newly characterized cell cycle specific nuclear autoantigen which is associated both with the centromeres and the nuclear matrix. It is expressed in low amounts in G0/G1-cells and accumulates in the nuclear matrix during S-phase with a maximum expression in G2/M-cells. In the present study we have investigated if p330d/CENP-F could be used as a marker for proliferation in different human malignancies. A flow cytometric method was developed by which p330d/CENP-F expression and DNA-content could be assessed on hematopoietic and solid tumors. Twenty-four different human hematopoietic malignancies, 12 breast cancers, and several cell lines were analyzed and the number of p330d/CENP-F positive cells and the S-phase fraction were determined. The percentage of p330d/CENP-F positive cells correlated with the fraction of S-phase cells in all human malignancies tested. Various cell lines revealed a similar cell cycle specific distribution. The association of p330d/CENP-F with the nuclear matrix facilitated the flow cytometric analysis of this protein due to its resistance to different preparation and fixation procedures. In summary, p330d/CENP-F seems to be a potentially valuable proliferation marker which can be applied to different tumors.

subject areas

  • Antigens, Neoplasm
  • Autoantigens
  • Biomarkers, Tumor
  • Breast Neoplasms
  • Cell Division
  • Chromosomal Proteins, Non-Histone
  • DNA, Neoplasm
  • Female
  • Flow Cytometry
  • Hematologic Neoplasms
  • Humans
  • Interphase
  • Male
  • Microfilament Proteins
  • Nuclear Matrix
  • S Phase
  • Tissue Fixation
  • Tumor Cells, Cultured
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Research

keywords

  • autoantibodies
  • cell cycle
  • centromere
  • flow cytometry
  • nuclear matrix
  • proliferation
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Identity

International Standard Serial Number (ISSN)

  • 0196-4763

Digital Object Identifier (DOI)

  • 10.1002/(sici)1097-0320(19960901)25:1<90::aid-cyto10>3.0.co;2-p

PubMed ID

  • 8875058
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Additional Document Info

start page

  • 90

end page

  • 98

volume

  • 25

issue

  • 1

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