Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Effects of chronic overexpression of interleukin-1 receptor antagonist in a model of permanent focal cerebral ischemia in mouse

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Oprica, M.
  • Van Dam, A. M.
  • Lundkvist, J.
  • Iverfeldt, K.
  • Winblad, B.
  • Bartfai, Tamas
  • Schultzberg, M.

publication date

  • 2004

journal

  • Acta Neuropathologica  Journal

abstract

  • Interleukin-1 receptor antagonist (IL-1ra) has been shown previously to have neuroprotective effects in animal models of stroke. The effects of chronic overexpression of human soluble IL-1ra (hsIL-1ra) were studied in a mouse model of permanent focal cerebral ischemia. A transgenic mouse strain (Tg hsIL-1ra+/-) has been developed using the promoter for glial fibrillary acidic protein (GFAP) to limit the overexpression to the CNS. Analysis of the neurological scores, infarct volume and edema formation revealed no differences between Tg hsIL-1ra+/- and wild-type (WT) mice. The cerebral ischemia resulted in pronounced astrocyte proliferation and microglial activation, as well as induction of inflammatory markers in both Tg hsIL-1ra+/- and WT mice, with no major differences between the two genotypes. Interestingly, hsIL-1ra expression in astrocytes was reduced in infarcted areas as compared to non-ischemic regions and sham-operated controls. In conclusion, transgenic overexpression of hsIL1-ra was not neuroprotective in this cerebral ischemia model, possibly due to insufficient levels for protection against the extensive lesion, or an up-regulation of compensatory inflammatory signals due to the lifetime blockade of IL-1 receptors.

subject areas

  • Animals
  • Brain Edema
  • Brain Infarction
  • Brain Ischemia
  • Caspase 1
  • Disease Models, Animal
  • Fluorescent Antibody Technique
  • Gene Expression Regulation
  • Glial Fibrillary Acidic Protein
  • Infarction, Middle Cerebral Artery
  • Interleukin 1 Receptor Antagonist Protein
  • Interleukin-1
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Neuroglia
  • Neurologic Examination
  • Regional Blood Flow
  • Sialoglycoproteins
scroll to property group menus

Research

keywords

  • caspase-1
  • cytokine
  • glia
  • infarct
  • inflammation
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0001-6322

Digital Object Identifier (DOI)

  • 10.1007/s00401-004-0868-5

PubMed ID

  • 15138779
scroll to property group menus

Additional Document Info

start page

  • 69

end page

  • 80

volume

  • 108

issue

  • 1

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support