Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Mus81 is essential for sister chromatid recombination at broken replication forks

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Roseaulin, L.
  • Yamada, Y.
  • Tsutsui, Y.
  • Russell, Paul
  • Iwasaki, H.
  • Arcangioli, B.

publication date

  • May 2008

journal

  • EMBO Journal  Journal

abstract

  • Recombination is essential for the recovery of stalled/collapsed replication forks and therefore for the maintenance of genomic stability. The situation becomes critical when the replication fork collides with an unrepaired single-strand break and converts it into a one-ended double-strand break. We show in fission yeast that a unique broken replication fork requires the homologous recombination (HR) enzymes for cell viability. Two structure-specific heterodimeric endonucleases participate in two different resolution pathways. Mus81/Eme1 is essential when the sister chromatid is used for repair; conversely, Swi9/Swi10 is essential when an ectopic sequence is used for repair. Consequently, the utilization of these two HR modes of resolution mainly relies on the ratio of unique and repeated sequences present in various eukaryotic genomes. We also provide molecular evidence for sister recombination intermediates. These findings demonstrate that Mus81/Eme1 is the dedicated endonuclease that resolves sister chromatid recombination intermediates during the repair of broken replication forks.

subject areas

  • Chromatids
  • Chromatin Immunoprecipitation
  • DNA Helicases
  • DNA Replication
  • DNA-Binding Proteins
  • Electrophoresis, Gel, Two-Dimensional
  • Endonucleases
  • Models, Genetic
  • Phenotype
  • Rad51 Recombinase
  • Recombination, Genetic
  • Schizosaccharomyces
  • Schizosaccharomyces pombe Proteins
scroll to property group menus

Research

keywords

  • recombination
  • replication collapse
  • sister chromatid recombination
scroll to property group menus

Identity

PubMed Central ID

  • PMC2374842

International Standard Serial Number (ISSN)

  • 0261-4189

Digital Object Identifier (DOI)

  • 10.1038/emboj.2008.65

PubMed ID

  • 18388861
scroll to property group menus

Additional Document Info

start page

  • 1378

end page

  • 1387

volume

  • 27

issue

  • 9

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support