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P{Switch}, a system for spatial and temporal control of gene expression in Drosophila melanogaster

Academic Article
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Overview

authors

  • Roman, G.
  • Endo, K.
  • Zong, L.
  • Davis, Ronald

publication date

  • October 2001

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • We have developed a method for turning on and off the expression of transgenes within Drosophila in both time and space. Two different enhancer detector elements carrying an RU486-inducible form of the yeast transcription factor GAL4 were constructed and used to generate enhancer detector lines. These lines were screened for RU486-inducible reporter gene expression in the adult head. We identified lines that exhibit inducible expression in many cell and tissue types, verifying that the elements respond to nearby enhancers. No expression was detected in the absence of the ligand. The P[Switch1] element responded to genomic enhancers less efficiently than P[Switch2] but produced more specific patterns of expression. Two P[Switch] lines were used to ablate fat body tissue in adult females through the induced expression of diphtheria toxin. These females were sterile, which correlates with fat body loss, and they died prematurely.

subject areas

  • Animals
  • Diphtheria Toxin
  • Drosophila melanogaster
  • Enhancer Elements, Genetic
  • Female
  • Gene Expression Regulation
  • Mifepristone
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Identity

PubMed Central ID

  • PMC60100

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.221303998

PubMed ID

  • 11675496
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Additional Document Info

start page

  • 12602

end page

  • 12607

volume

  • 98

issue

  • 22

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