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Lack of N-regions in fetal and neonatal mouse immunoglobulin V-D-J junctional sequences

Academic Article
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Overview

authors

  • Feeney, Ann

publication date

  • November 1990

journal

  • Journal of Experimental Medicine  Journal

abstract

  • Much of T and B lymphocyte receptor diversity derives from the addition of nontemplated N regions at the junctions of receptor gene elements, although fetal T cells expressing gamma/delta receptors lack N regions. I have sequenced immunoglobulin H chain variable regions of PCR-amplified DNA and cDNA from fetal and newborn mouse liver and spleen cells. These sequences showed an absence of N regions. Only 1/87 DNA sequences and 17/146 RNA sequences contained N regions, in striking contrast to adult Ig sequences. These data show that N region insertion is a developmentally regulated process in B cells as well as in T cells, and demonstrate that receptor diversity in neonatal B cells is limited by the absence of N regions as well as by biased usage of Vh genes.

subject areas

  • Animals
  • Animals, Newborn
  • Base Sequence
  • DNA
  • DNA Transposable Elements
  • Fetus
  • Gene Rearrangement
  • Genes, Immunoglobulin
  • Immunoglobulin Joining Region
  • Liver
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • RNA
  • Spleen
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Identity

International Standard Serial Number (ISSN)

  • 0022-1007

Digital Object Identifier (DOI)

  • 10.1084/jem.172.5.1377

PubMed ID

  • 1700054
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Additional Document Info

start page

  • 1377

end page

  • 1390

volume

  • 172

issue

  • 5

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