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Increasing the chemical potential of the germ-line antibody repertoire

Academic Article
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Overview

authors

  • Sarvetnick, N.
  • Gurushanthaiah, D.
  • Han, N. H.
  • Prudent, J.
  • Schultz, Peter
  • Lerner, Richard

publication date

  • May 1993

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • To augment the chemical potential of the immunological repertoire, a metal ion-binding light chain has been introduced into the murine genome. Mice containing the transgene were subsequently immunized with a fluorescein conjugate. The transgenic light chain was found at a high frequency in the anti-fluorescein memory B-cell compartment. This general method should be applicable to other cofactors and small molecules and should lead to generation of antibodies with unique catalytic activities.

subject areas

  • Animals
  • Antibody Formation
  • B-Lymphocytes
  • Base Sequence
  • Crosses, Genetic
  • Exons
  • Hybridomas
  • Immunoglobulin kappa-Chains
  • Liver
  • Mice
  • Mice, Inbred Strains
  • Mice, Transgenic
  • Molecular Sequence Data
  • Mutagenesis, Site-Directed
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Spleen
  • Tumor Cells, Cultured
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Research

keywords

  • CATALYSIS
  • COFACTORS
  • COORDINATION COMPLEX
  • TRANSGENIC MICE
  • TRANSITION METALS
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Identity

PubMed Central ID

  • PMC46435

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.90.9.4008

PubMed ID

  • 8483917
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Additional Document Info

start page

  • 4008

end page

  • 4011

volume

  • 90

issue

  • 9

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