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Interferon prevents formation of replication-competent hepatitis B virus RNA-containing nucleocapsids

Academic Article
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Overview

authors

  • Wieland, Stefan
  • Eustaquio, A.
  • Whitten-Bauer, C.
  • Boyd, B.
  • Chisari, Francis

publication date

  • July 2005

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • We have previously shown that IFN-beta inhibits hepatitis B virus (HBV) replication by noncytolytic mechanisms that either destabilize pregenomic (pg)RNA-containing capsids or prevent their assembly. Using immortalized murine hepatocyte cell lines stably transfected with a doxycycline (dox)-inducible HBV replication system, we now show that replication-competent pgRNA-containing capsids are not produced when the cells are pretreated with IFN-beta before HBV expression is induced with dox. Furthermore, the turnover rate of preformed HBV RNA-containing capsids is not changed in the presence of IFN-beta or IFN-gamma under conditions in which further pgRNA synthesis is inhibited by dox removal. In summary, these results demonstrate that types 1 and 2 IFN activate hepatocellular mechanism(s) that prevent the formation of replication-competent HBV capsids and, thereby, inhibit HBV replication.

subject areas

  • Animals
  • Blotting, Southern
  • Capsid
  • Cell Line
  • Doxycycline
  • Hepatitis B virus
  • Interferon-beta
  • Mice
  • Plasmids
  • Polymerase Chain Reaction
  • RNA
  • Virus Replication
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Research

keywords

  • antiviral
  • beta
  • interferon gamma
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Identity

PubMed Central ID

  • PMC1175012

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0504273102

PubMed ID

  • 15994231
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Additional Document Info

start page

  • 9913

end page

  • 9917

volume

  • 102

issue

  • 28

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