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Targeted disruption of the galectin-3 gene results in attenuated peritoneal inflammatory responses

Academic Article
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Overview

authors

  • Hsu, D. K.
  • Yang, R. Y.
  • Pan, Z. X.
  • Lu, L.
  • Salomon, Daniel
  • Fung-Leung, W. P.
  • Liu, F. T.

publication date

  • March 2000

journal

  • American Journal of Pathology  Journal

abstract

  • Galectin-3 is a member of a growing family of beta-galactoside-binding animal lectins. Previous studies have demonstrated a variety of biological activities for this protein in vitro, including activation of cells, modulation of cell adhesion, induction of pre-mRNA splicing, and regulation of apoptosis. To assist in fully elucidating the physiological and pathological functions of this protein, we have generated galectin-3-deficient (gal3(-/-)) mice by targeted interruption of the galectin-3 gene. Gal3(-/-) mice consistently developed fewer inflammatory cell infiltrations in the peritoneal cavities than the wild-type (gal3(+/+)) mice in response to thioglycollate broth treatment, mainly due to lower numbers of macrophages. Also, when compared to cells from gal3(+/+) mice, thioglycollate-elicited inflammatory cells from gal3(-/-) mice exhibited significantly lower levels of NF-kappaB response. In addition, dramatically different cell-spreading phenotypes were observed in cultured macrophages from the two genotypes. Whereas macrophages from gal3(+/+) mice exhibited well spread out morphology, those from gal3(-/-) mice were often spindle-shaped. Finally, we found that peritoneal macrophages from gal3(-/-) mice were more prone to undergo apoptosis than those from gal3(+/+) mice when treated with apoptotic stimuli, suggesting that expression of galectin-3 in inflammatory cells may lead to longer cell survival, thus prolonging inflammation. These results strongly support galectin-3 as a positive regulator of inflammatory responses in the peritoneal cavity.

subject areas

  • Animals
  • Antigens, Differentiation
  • Apoptosis
  • Blotting, Southern
  • Cell Adhesion
  • Cell Count
  • Cells, Cultured
  • DNA
  • Disease Models, Animal
  • Galectin 3
  • Gene Targeting
  • Homozygote
  • Lectins
  • Leukocytes
  • Lymphocyte Activation
  • Macrophages, Peritoneal
  • Mice
  • Mice, Inbred C57BL
  • Mice, Knockout
  • NF-kappa B
  • Peritonitis
  • Polymerase Chain Reaction
  • Thioglycolates
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Identity

International Standard Serial Number (ISSN)

  • 0002-9440

Digital Object Identifier (DOI)

  • 10.1016/s0002-9440(10)64975-9

PubMed ID

  • 10702423
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Additional Document Info

start page

  • 1073

end page

  • 1083

volume

  • 156

issue

  • 3

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