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A double monoclonal IgG1k and IgG2k in a single myeloma patient - variation in clonal products and therapeutic responses

Academic Article
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Overview

authors

  • Goni, F.
  • Chuba, J.
  • Buxbaum, Joel
  • Frangione, B.

publication date

  • January 1988

journal

  • Journal of Immunology  Journal

abstract

  • Two electrophoretically homogeneous immunoglobulins were detected in the serum of a patient with multiple myeloma. The heavy chains were of different classes (gamma 1 and gamma 2). The light chains of both were kappa, but had different electrophoretic mobilities on polyacrylamide gels. Amino acid sequence analysis, carbohydrate determinations, and biosynthetic experiments indicated that the difference seen in their electrophoretic mobility was due to the glycosylation of one but not the other kappa-chain. The primary structure of both chains demonstrated that they both used a V kappa 1 and a J kappa 2 gene segment and the same C kappa allele, Km(1,2), and that both contained the same junctional three amino acid deletion. However, they varied by 19 amino acids in the first 94 amino terminal residues encoded for by the V kappa gene, with some of the substitutions requiring two base changes in the appropriate codons. Moreover, the malignant "clones" producing the two proteins differed in their responses to chemotherapy. These data indicate that, although the two clones producing the serum proteins were different at the time of study, they may have arisen from the same precursor clone.

subject areas

  • Amino Acid Sequence
  • Antibodies, Monoclonal
  • Base Sequence
  • Bone Marrow
  • Clone Cells
  • Electrophoresis, Agar Gel
  • Humans
  • Immunoglobulin G
  • Immunoglobulin Light Chains
  • Immunoglobulin kappa-Chains
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Molecular Weight
  • Multiple Myeloma
  • Peptide Fragments
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 3121749
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Additional Document Info

start page

  • 551

end page

  • 557

volume

  • 140

issue

  • 2

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