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Assignment of endogenous substrates to enzymes by global metabolite profiling

Academic Article
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Overview

authors

  • Saghatelian, Alan
  • Trauger, S. A.
  • Want, E. J.
  • Hawkins, E. G.
  • Siuzdak, Gary
  • Cravatt, Benjamin

publication date

  • November 2004

journal

  • Biochemistry  Journal

abstract

  • Enzymes regulate biological processes through the conversion of specific substrates to products. Therefore, of fundamental interest for every enzyme is the elucidation of its natural substrates. Here, we describe a general strategy for identifying endogenous substrates of enzymes by untargeted liquid chromatography-mass spectrometry (LC-MS) analysis of tissue metabolomes from wild-type and enzyme-inactivated organisms. We use this method to discover several brain lipids regulated by the mammalian enzyme fatty acid amide hydrolase (FAAH) in vivo, including known signaling molecules (e.g., the endogenous cannabinoid anandamide) and a novel family of nervous system-enriched natural products, the taurine-conjugated fatty acids. Remarkably, the relative hydrolytic activity that FAAH exhibited for lipid metabolites in vitro was not predictive of the identity of specific FAAH substrates in vivo. Thus, global metabolite profiling establishes unanticipated connections between the proteome and metabolome that enable assignment of an enzyme's unique biochemical functions in vivo.

subject areas

  • Amidohydrolases
  • Animals
  • Brain
  • Chromatography, Liquid
  • Ethanolamines
  • Fatty Acids
  • Hydrolysis
  • Mass Spectrometry
  • Mice
  • Mice, Knockout
  • Predictive Value of Tests
  • Spinal Cord
  • Substrate Specificity
  • Taurine
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Identity

International Standard Serial Number (ISSN)

  • 0006-2960

Digital Object Identifier (DOI)

  • 10.1021/bi0480335

PubMed ID

  • 15533037
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Additional Document Info

start page

  • 14332

end page

  • 14339

volume

  • 43

issue

  • 45

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