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Immunocytochemical characterization of human NOR-90 (upstream binding factor) and associated antigens reactive with autoimmune sera. Two MR forms of NOR-90/hUBF autoantigens

Academic Article
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Overview

authors

  • Imai, H.
  • Fritzler, M. J.
  • Neri, R.
  • Bombardieri, S.
  • Tan, Eng
  • Chan, E. K. L.

publication date

  • March 1994

journal

  • Molecular Biology Reports  Journal

abstract

  • The 90-kDa nucleolus organizer region autoantigen (NOR-90) was previously shown to be identical to the human upstream binding factor (hUBF) and composed of two Mr forms. In this study, thirteen human anti-NOR-90/hUBF autoimmune sera were used to further characterize NOR-90/hUBF and its associated autoantigens. Nucleolar and nucleoplasmic staining of interphase cells and NOR staining in mitosis were observed with all sera by immunofluorescence. All sera showed equal reactivity with both high and low Mr forms in Western blotting and immunoprecipitation, suggesting that the cellular content and distribution for both Mr forms were approximately equal. Using extracts of [35S]methionine- and [32P]orthophosphate-labeled cells, phosphorylated and nonphosphorylated NOR-90/hUBF were identified for both Mr forms and these two populations were recognized by human autoantibodies. In immunoprecipitation analyses, the nonphosphorylated population was readily extracted while the phosphorylated population was tightly bound. Clinical data were available for 8 patients in whom anti-NOR-90/hUBF autoantibodies were present. They had diverse diagnoses including SLE, rheumatoid arthritis and malignancies. Although only one patient was diagnosed as scleroderma, Raynaud's phenomenon was observed in 4 of the 8 patients. Interestingly, one NOR-90/hUBF serum was shown to contain additional antibodies to RNA polymerases I and II.

subject areas

  • Adult
  • Aged
  • Arthritis, Rheumatoid
  • Autoantibodies
  • Autoantigens
  • Autoimmune Diseases
  • Autoimmunity
  • DNA-Binding Proteins
  • Female
  • Humans
  • Immunohistochemistry
  • Lupus Erythematosus, Systemic
  • Male
  • Middle Aged
  • Neoplasms
  • Nucleolus Organizer Region
  • Pol1 Transcription Initiation Complex Proteins
  • Scleroderma, Systemic
  • Transcription Factors
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Research

keywords

  • AUTOANTIBODY
  • HUMAN UPSTREAM BINDING FACTOR
  • NUCLEOLUS ORGANIZER REGION
  • RNA POLYMERASE I
  • RNA POLYMERASE II
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Identity

International Standard Serial Number (ISSN)

  • 0301-4851

Digital Object Identifier (DOI)

  • 10.1007/bf00997157

PubMed ID

  • 8072492
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Additional Document Info

start page

  • 115

end page

  • 124

volume

  • 19

issue

  • 2

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