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Molecular architecture of the human pre-mRNA 3' processing complex

Academic Article
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Overview

authors

  • Shi, Y. S.
  • Di Giammartino, D. C.
  • Taylor, D.
  • Sarkeshik, A.
  • Rice, W. J.
  • Yates III, John
  • Frank, J.
  • Manley, J. L.

publication date

  • February 2009

journal

  • Molecular Cell  Journal

abstract

  • Pre-mRNA 3' end formation is an essential step in eukaryotic gene expression. Over half of human genes produce alternatively polyadenylated mRNAs, suggesting that regulated polyadenylation is an important mechanism for posttranscriptional gene control. Although a number of mammalian mRNA 3' processing factors have been identified, the full protein composition of the 3' processing machinery has not been determined, and its structure is unknown. Here we report the purification and subsequent proteomic and structural characterization of human mRNA 3' processing complexes. Remarkably, the purified 3' processing complex contains approximately 85 proteins, including known and new core 3' processing factors and over 50 proteins that may mediate crosstalk with other processes. Electron microscopic analyses show that the core 3' processing complex has a distinct "kidney" shape and is approximately 250 A in length. Together, our data has revealed the complexity and molecular architecture of the pre-mRNA 3' processing complex.

subject areas

  • 3' Untranslated Regions
  • Humans
  • Polyadenylation
  • Proteome
  • Proteomics
  • RNA 3' End Processing
  • RNA Precursors
  • RNA, Messenger
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Identity

PubMed Central ID

  • PMC2946185

International Standard Serial Number (ISSN)

  • 1097-2765

Digital Object Identifier (DOI)

  • 10.1016/j.molcel.2008.12.028

PubMed ID

  • 19217410
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Additional Document Info

start page

  • 365

end page

  • 376

volume

  • 33

issue

  • 3

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