Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

A monoclonal antibody against the nuclear pore complex inhibits nucleocytoplasmic transport of protein and RNA in viv

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Featherstone, C.
  • Darby, M. K.
  • Gerace, Larry

publication date

  • October 1988

journal

  • Journal of Cell Biology  Journal

abstract

  • A monoclonal antibody that reacts with proteins in the nuclear pore complex of rat liver (Snow, C. M., A. Senior, and L. Gerace. 1987. J. Cell Biol. 104:1143-1156) has been shown to cross react with similar components in Xenopus oocytes, as determined by immunofluorescence microscopy and immunoblotting. We have microinjected the antibody into oocytes to study the possible role of these polypeptides in nucleocytoplasmic transport. The antibody inhibits import of a large nuclear protein, nucleoplasmin, in a time- and concentration-dependent manner. It also inhibits export of 5S ribosomal RNA and mature tRNA, but has no effect on transcription or intranuclear tRNA processing. The antibody does not affect the rate of diffusion into the nucleus of two small proteins, myoglobin and ovalbumin, indicating that antibody binding does not result in occlusion of the channel for diffusion. This suggests that inhibition of protein and RNA transport occurs by binding of the antibody at or near components of the pore that participate in mediated transport.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Biological Transport
  • Blotting, Western
  • Diffusion
  • Fluorescent Antibody Technique
  • Immunologic Techniques
  • Nuclear Envelope
  • Oocytes
  • Proteins
  • RNA
  • Xenopus laevis
scroll to property group menus

Identity

PubMed Central ID

  • PMC2115252

International Standard Serial Number (ISSN)

  • 0021-9525

Digital Object Identifier (DOI)

  • 10.1083/jcb.107.4.1289

PubMed ID

  • 2459127
scroll to property group menus

Additional Document Info

start page

  • 1289

end page

  • 1297

volume

  • 107

issue

  • 4

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support