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Structural basis for subversion of cellular control mechanisms by the adenoviral e1a oncoprotein

Academic Article
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Overview

authors

  • Ferreon, J. C.
  • Martinez-Yamout, M. A.
  • Dyson, Jane
  • Wright, Peter

publication date

  • August 2009

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • The adenovirus early region 1A (E1A) oncoprotein mediates cell transformation by deregulating host cellular processes and activating viral gene expression by recruitment of cellular proteins that include cyclic-AMP response element binding (CREB) binding protein (CBP)/p300 and the retinoblastoma protein (pRb). While E1A is capable of independent interaction with CBP/p300 or pRb, simultaneous binding of both proteins is required for maximal biological activity. To obtain insights into the mechanism by which E1A hijacks the cellular transcription machinery by competing with essential transcription factors for binding to CBP/p300, we have determined the structure of the complex between the transcriptional adaptor zinc finger-2 (TAZ2) domain of CBP and the conserved region-1 (CR1) domain of E1A. The E1A CR1 domain is unstructured in the free state and upon binding folds into a local helical structure mediated by an extensive network of intermolecular hydrophobic contacts. By NMR titrations, we show that E1A efficiently competes with the N-terminal transactivation domain of p53 for binding to TAZ2 and that pRb interacts with E1A at 2 independent sites located in CR1 and CR2. We show that pRb and the CBP TAZ2 domain can bind simultaneously to the CR1 site of E1A to form a ternary complex and propose a structural model for the pRb:E1A:CBP complex on the basis of published x-ray data for homologous binary complexes. These observations reveal the molecular basis by which E1A inhibits p53-mediated transcriptional activation and provide a rationale for the efficiency of cellular transformation by the adenoviral E1A oncoprotein.

subject areas

  • Adenovirus E1A Proteins
  • Amino Acid Sequence
  • Animals
  • Binding, Competitive
  • Magnetic Resonance Spectroscopy
  • Mice
  • Models, Molecular
  • Molecular Sequence Data
  • Multiprotein Complexes
  • Oncogene Proteins
  • Protein Binding
  • Protein Interaction Mapping
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Retinoblastoma Protein
  • Tumor Suppressor Protein p53
  • p300-CBP Transcription Factors
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Research

keywords

  • CBP/p300
  • NMR
  • protein structure
  • retinoblastoma protein
  • transcriptional coactivator
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Identity

PubMed Central ID

  • PMC2726373

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0906770106

PubMed ID

  • 19651603
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Additional Document Info

start page

  • 13260

end page

  • 13265

volume

  • 106

issue

  • 32

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