Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Differential kinetics of transcription complex assembly distinguish oocyte and somatic 5S RNA genes of Xenopus

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • McBryant, S. J.
  • Gottesfeld, Joel

publication date

  • 1997

journal

  • Gene Expression  Journal

abstract

  • Differential transcription of the Xenopus gene families encoding the oocyte and somatic 5S ribosomal RNAs can be reproduced in vitro with cell-free extracts prepared from Xenopus oocytes and unfertilized eggs. The transcriptional activities of these genes as assayed in these in vitro systems are a consequence of large differences in the rates of assembly of active transcription complexes. The somatic 5S genes sequester limiting transcription factors much more rapidly than the corresponding oocyte 5S genes and, as a consequence, are far more active. However, once transcription complexes are formed, these complexes are stable on both of these genes. Previous studies have established that transcription factors IIIA and IIIC are sufficient to form a stable protein-DNA complex on the somatic 5S gene. The rate of formation of the stable TFIIIA+C complex for the oocyte gene is far slower than that for the somatic 5S gene. Insertion of the DNA binding site for TFIIIC2 (the B-block promoter element from tRNA genes) into the 3' flanking region of a synthetic oocyte 5S gene increases the transcription efficiency and rate of transcription complex assembly of this gene relative to the parent gene lacking the B-block element. Our results support a model in which competition for limiting transcription factors plays a pivotal role in establishing differential transcription of the two classes of 5S genes during early embryogenesis.

subject areas

  • Animals
  • Cell Extracts
  • Chromatin
  • DNA, Ribosomal
  • DNA-Binding Proteins
  • Kinetics
  • Oocytes
  • RNA, Ribosomal, 5S
  • Transcription Factor TFIIIA
  • Transcription Factors
  • Transcription Factors, TFIII
  • Transcription, Genetic
  • Xenopus laevis
scroll to property group menus

Research

keywords

  • 5S RNA genes
  • Pol III transcription
  • TFIIIA
  • TFIIIC
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 1052-2166

PubMed ID

  • 9495319
scroll to property group menus

Additional Document Info

start page

  • 387

end page

  • 399

volume

  • 6

issue

  • 6

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support