Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Maturation dynamics of a viral capsid: Visualization of transitional intermediate states

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Lata, R.
  • Conway, J. F.
  • Cheng, N. Q.
  • Duda, R. L.
  • Hendrix, R. W.
  • Wikoff, W. R.
  • Johnson Jr., John
  • Tsuruta, H.
  • Steven, A. C.

publication date

  • January 2000

journal

  • Cell  Journal

abstract

  • Typical of DNA bacteriophages and herpesviruses, HK97 assembles in two stages: polymerization and maturation. First, capsid protein polymerizes into closed shells; then, these precursors mature into larger, stabler particles. Maturation is initiated by proteolysis, producing a metastable particle primed for expansion-the major structural transition. We induced expansion in vitro by acidic pH and monitored the resulting changes by time-resolved X-ray diffraction and cryo-electron microscopy. The transition, which is not synchronized over the population, proceeds in a series of stochastically triggered subtransitions. Three distinct intermediates were identified, which are comparable to transitional states in protein folding. The intermediates' structures reveal the molecular events occurring during expansion. Integrated into a movie (see Dynamic Visualization below), they show capsid maturation as a dynamic process.

subject areas

  • Acids
  • Bacteriophages
  • Capsid
  • Cryoelectron Microscopy
  • Endopeptidases
  • Hydrogen-Ion Concentration
  • Kinetics
  • Models, Chemical
  • Plasmids
  • Protein Folding
  • Scattering, Radiation
  • Viral Core Proteins
  • X-Ray Diffraction
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0092-8674

Digital Object Identifier (DOI)

  • 10.1016/s0092-8674(00)81563-9

PubMed ID

  • 10660048
scroll to property group menus

Additional Document Info

start page

  • 253

end page

  • 263

volume

  • 100

issue

  • 2

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support