Isolation of highly malignant Thy-1-positive revertants from cultured cloned Thy-1-negative lymphoma-cells of low tumorigenicity

Academic Article
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publication date

  • 1984

abstract

  • We have used a positive immunoselection method to isolate a Thy-1-positive revertant of a Thy-1-negative line. The technique makes use of the ability of surface bound antigen-antibody complexes containing the enzyme catalase to protect cells from the toxic effects of hydrogen peroxide. The revertant cells produce a Thy-1 molecule indistinguishable from that produced by the original parent line, and they resemble that line in being far more tumorigenic than the Thy-1-negative variant.