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Isolation of highly malignant Thy-1-positive revertants from cultured cloned Thy-1-negative lymphoma-cells of low tumorigenicity

Academic Article
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Overview

authors

  • Basch, R. S.
  • Lakow, E.
  • Teng, M.
  • Buxbaum, Joel

publication date

  • 1984

journal

  • Somatic Cell and Molecular Genetics  Journal

abstract

  • We have used a positive immunoselection method to isolate a Thy-1-positive revertant of a Thy-1-negative line. The technique makes use of the ability of surface bound antigen-antibody complexes containing the enzyme catalase to protect cells from the toxic effects of hydrogen peroxide. The revertant cells produce a Thy-1 molecule indistinguishable from that produced by the original parent line, and they resemble that line in being far more tumorigenic than the Thy-1-negative variant.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antigen-Antibody Complex
  • Antigens, Surface
  • Antigens, Thy-1
  • Catalase
  • Cell Line
  • Cell Membrane
  • Clone Cells
  • Fluorescent Antibody Technique
  • Lymphoma
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mutation
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Identity

International Standard Serial Number (ISSN)

  • 0740-7750

Digital Object Identifier (DOI)

  • 10.1007/bf01534471

PubMed ID

  • 6142539
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Additional Document Info

start page

  • 37

end page

  • 44

volume

  • 10

issue

  • 1

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