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Human recombinant erythropoietin promotes differentiation of murine megakaryocytes in vitro

Academic Article
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Overview

authors

  • Ishibashi, T.
  • Koziol, James
  • Burstein, S. A.

publication date

  • January 1987

journal

  • Journal of Clinical Investigation  Journal

abstract

  • To determine if erythropoietin affects megakaryocytopoiesis, we measured acetylcholinesterase (AchE) activity, a marker of the murine megakaryocytic lineage, after the addition of human recombinant erythropoietin to serumless murine bone marrow cultures. Erythropoietin increased AchE activity substantially. Moreover, when the hormone was added to serumless cultures of 426 isolated single megakaryocytes derived from megakaryocytic colonies, erythropoietin induced a significant increase in the diameters of these cells. From a Bayesian analysis of the likelihood that some megakaryocytes increased in DNA content during the culture period, we estimate that 61% of the cells increased in ploidy. These data indicate that the action of erythropoietin is not restricted to the erythroid lineage.

subject areas

  • Acetylcholinesterase
  • Animals
  • Cell Differentiation
  • Cells, Cultured
  • Erythropoietin
  • Hematopoiesis
  • Humans
  • Megakaryocytes
  • Mice
  • Recombinant Proteins
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Identity

PubMed Central ID

  • PMC424045

International Standard Serial Number (ISSN)

  • 0021-9738

Digital Object Identifier (DOI)

  • 10.1172/jci112796

PubMed ID

  • 3793927
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Additional Document Info

start page

  • 286

end page

  • 289

volume

  • 79

issue

  • 1

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