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Constitutively unmasked cd22 on b cells of st6gal i knockout mice: Novel sialoside probe for murine cd22

Academic Article
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Overview

authors

  • Collins, B. E.
  • Blixt, O.
  • Bovin, N. V.
  • Danzer, C. P.
  • Chui, D.
  • Marth, J. D.
  • Nitschke, L.
  • Paulson, James

publication date

  • September 2002

journal

  • Glycobiology  Journal

abstract

  • The interaction of CD22 with glycoprotein ligands bearing the Siaalpha2,6Gal-R sequence is believed to modulate its function as a regulator of B cell signaling. Although a commercial sialoside-polyacrylamide (PAA) probe, NeuAc- alpha2,6Gal-PAA, has facilitated studies on ligand binding by human CD22, murine CD22 binds instead with high affinity to NeuGcalpha2,6Gal-R. A multivalent probe with this sequence was constructed to facilitate investigations of ligand binding in CD22 function using genetically defined murine models. The probe is based on the sialoside-PAA platform, which is then biotinylated for easy detection. A series of sialoside probes were constructed with two different length linker arms between the sialoside and the backbone and three different sialoside to PAA molar ratios. The NeuGcalpha2,6Gal-PAA probe is specific for CD22: it binds to sialidase-treated B cells of wild-type mice but not B cells of CD22-null mice. Additionally, because the probe only binds to sialidase-treated wild-type cells, it confirms that CD22 is constitutively "masked" on most B cells from wild-type mice by binding to ligands in cis. In contrast, the probe bound equally well to native or sialidase-treated B cells from the immunocompromised ligand-deficient ST6Gal I knockout mice, demonstrating that CD22 is constitutively "unmasked" in these cells.

subject areas

  • Animals
  • Antigens, CD
  • Antigens, Differentiation, B-Lymphocyte
  • B-Lymphocytes
  • Cell Adhesion Molecules
  • Lectins
  • Mice
  • Mice, Knockout
  • Molecular Probes
  • Sialic Acid Binding Ig-like Lectin 2
  • Sialyltransferases
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Research

keywords

  • CD22
  • NeuGc
  • ST6Gal I
  • Siglec
  • probe
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Identity

International Standard Serial Number (ISSN)

  • 0959-6658

Digital Object Identifier (DOI)

  • 10.1093/glycob/cwf067

PubMed ID

  • 12213789
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Additional Document Info

start page

  • 563

end page

  • 571

volume

  • 12

issue

  • 9

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