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Characterization of the human submandibular/sublingual saliva glycoproteome using lectin affinity chromatography coupled to multidimensional protein identification technology

Academic Article
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Overview

authors

  • Gonzalez-Begne, M.
  • Lu, B. W.
  • Liao, L. J.
  • Xu, T.
  • Bedi, G.
  • Melvin, J. E.
  • Yates III, John

publication date

  • November 2011

journal

  • Journal of Proteome Research  Journal

abstract

  • In-depth analysis of the salivary proteome is fundamental to understanding the functions of salivary proteins in the oral cavity and to reveal disease biomarkers involved in different pathophysiological conditions, with the ultimate goal of improving patient diagnosis and prognosis. Submandibular and sublingual glands contribute saliva rich in glycoproteins to the total saliva output, making them valuable sources for glycoproteomic analysis. Lectin-affinity chromatography coupled to mass spectrometry-based shotgun proteomics was used to explore the submandibular/sublingual (SM/SL) saliva glycoproteome. A total of 262 N- and O-linked glycoproteins were identified by multidimensional protein identification technology (MudPIT). Only 38 were previously described in SM and SL salivas from the human salivary N-linked glycoproteome, while 224 were unique. Further comparison analysis with SM/SL saliva of the human saliva proteome, revealed 125 glycoproteins not formerly reported in this secretion. KEGG pathway analyses demonstrated that many of these glycoproteins are involved in processes such as complement and coagulation cascades, cell communication, glycosphingolipid biosynthesis neo-lactoseries, O-glycan biosynthesis, glycan structures-biosynthesis 2, starch and sucrose metabolism, peptidoglycan biosynthesis or others pathways. In summary, lectin-affinity chromatography coupled to MudPIT mass spectrometry identified many novel glycoproteins in SM/SL saliva. These new additions to the salivary proteome may prove to be a critical step for providing reliable biomarkers in the diagnosis of a myriad of oral and systemic diseases.

subject areas

  • Carrier Proteins
  • Chromatography, Affinity
  • Glycoproteins
  • Humans
  • Lectins
  • Male
  • Middle Aged
  • Proteome
  • Saliva
  • Sublingual Gland
  • Submandibular Gland
  • Tandem Mass Spectrometry
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Research

keywords

  • MudPIT
  • biomarkers
  • glycoproteins
  • lectin-affinity chromatography
  • submandibular/sublingual saliva
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Identity

PubMed Central ID

  • PMC3279195

International Standard Serial Number (ISSN)

  • 1535-3893

Digital Object Identifier (DOI)

  • 10.1021/pr200505t

PubMed ID

  • 21936497
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Additional Document Info

start page

  • 5031

end page

  • 5046

volume

  • 10

issue

  • 11

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