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Induction of antiviral antibodies by DNA immunization requires neither perforin-mediated nor cd8(+)-t-cell-mediated lysis of antigen-expressing cells

Academic Article
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Overview

authors

  • Hassett, D. E.
  • Zhang, J.
  • Whitton, J. Lindsay

publication date

  • September 1999

journal

  • Journal of Virology  Journal

abstract

  • DNA immunization induces antibodies to the encoded protein, which indicates that the protein must gain access to the extracellular milieu, allowing it to interact with naïve B lymphocytes. It has been suggested that antigen release may be effected by cytotoxic-T-lymphocyte-mediated lysis of transfected antigen-expressing cells; this might be particularly important for the induction of responses to a noncytopathic, cytosolic protein. Here we show that the induction of antibody responses to one such DNA-encoded protein required neither perforin nor CD8(+) T cells. In addition, there was no skewing of the immunoglobulin G isotypes in the absence of perforin.

subject areas

  • Animals
  • Antibodies, Viral
  • CD8-Positive T-Lymphocytes
  • Cytotoxicity, Immunologic
  • DNA, Viral
  • Immunoglobulin G
  • Immunoglobulin Isotypes
  • Lymphocytic choriomeningitis virus
  • Membrane Glycoproteins
  • Mice
  • Mice, Inbred C57BL
  • Nucleoproteins
  • Perforin
  • Pore Forming Cytotoxic Proteins
  • Vaccines, DNA
  • Viral Proteins
  • Viral Vaccines
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Identity

PubMed Central ID

  • PMC104319

International Standard Serial Number (ISSN)

  • 0022-538X

PubMed ID

  • 10438882
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Additional Document Info

start page

  • 7870

end page

  • 7873

volume

  • 73

issue

  • 9

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