Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Yych regulates the activity of the essential yycfg two-component system in bacillus subtilis

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Szurmant, Hendrik
  • Nelson, K.
  • Kim, E. J.
  • Perego, Marta
  • Hoch, James

publication date

  • 2005

journal

  • Journal of Bacteriology  Journal

abstract

  • Of the numerous two-component signal transduction systems found in bacteria, only a very few have proven to be essential for cell viability. Among these is the YycF (response regulator)-YycG (histidine kinase) system, which is highly conserved in and specific to the low-G+C content gram-positive bacteria. Given the pathogenic nature of several members of this class of bacteria, the YycF-YycG system has been suggested as a prime antimicrobial target. In an attempt to identify genes involved in regulation of this two-component system, a transposon mutagenesis study was designed to identify suppressors of a temperature-sensitive YycF mutant in Bacillus subtilis. Suppressors could be identified, and the prime target was the yycH gene located adjacent to yycG and within the same operon. A lacZ reporter assay revealed that YycF-regulated gene expression was elevated in a yycH strain, whereas disruption of any of the three downstream genes within the operon, yycI, yycJ, and yycK, showed no such effect. The concentrations of both YycG and YycF, assayed immunologically, remained unchanged between the wild-type and the yycH strain as determined by immunoassay. Alkaline phosphatase fusion studies showed that YycH is located external to the cell membrane, suggesting that it acts in the regulation of the sensor domain of the YycG sensor histidine kinase. The yycH strain showed a characteristic cell wall defect consistent with the previously suggested notion that the YycF-YycG system is involved in regulating cell wall homeostasis and indicating that either up- or down-regulation of YycF activity affects this homeostatic mechanism.

subject areas

  • Bacillus subtilis
  • Bacterial Proteins
  • Cell Wall
  • Gene Expression Regulation, Bacterial
  • Genes, Bacterial
  • Genes, Regulator
  • Homeostasis
  • Operon
  • Protein Kinases
  • Signal Transduction
  • Temperature
  • Trans-Activators
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.187.15.5419-5426.2005

PubMed ID

  • 16030236
scroll to property group menus

Additional Document Info

start page

  • 5419

end page

  • 5426

volume

  • 187

issue

  • 15

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support