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Hepatitis-B virus nucleocapsid particles do not cross the hepatocyte nuclear-membrane in transgenic mice

Academic Article
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Overview

authors

  • Guidotti, Luca
  • Martinez, V.
  • Loh, Y. T.
  • Rogler, C. E.
  • Chisari, Francis

publication date

  • 1994

journal

  • Journal of Virology  Journal

abstract

  • Transgenic mice that express the hepatitis B virus core protein were used to examine factors that influence the intracellular localization of nucleocapsid particles in the primary hepatocyte in vivo. In this model, viral nucleocapsid particles are strictly localized to the nucleus of the hepatocyte except when the nuclear membrane dissolves during cell division, at which time they enter the cytoplasm. The cytoplasmic nucleocapsid particles do not reenter the nucleus, however, when the nuclear membrane re-forms after cell division. The data support the notion that nucleocapsid particles can form de novo within the nucleus, and they suggest that performed nucleocapsid particles cannot be transported across the intact nuclear membrane in either direction. The results imply that nucleocapsid disassembly is probably required for entry of the hepadnaviral genome into the nucleus, and they question the role of the intranuclear viral nucleocapsid particle during the viral life cycle.

subject areas

  • Animals
  • Base Sequence
  • Capsid
  • Cell Compartmentation
  • Cell Cycle
  • Cell Nucleus
  • DNA Primers
  • Hepatitis B Core Antigens
  • Hepatitis B virus
  • Liver
  • Mice
  • Mice, Transgenic
  • Mitosis
  • Molecular Sequence Data
  • RNA, Viral
  • Time Factors
  • Viral Core Proteins
  • Virus Replication
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Identity

PubMed Central ID

  • PMC236947

International Standard Serial Number (ISSN)

  • 0022-538X

PubMed ID

  • 8057429
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Additional Document Info

start page

  • 5469

end page

  • 5475

volume

  • 68

issue

  • 9

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