Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Muscarinic receptors and receptor-mediated actions on rat thymocytes

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Maslinski, W.
  • Kullberg, M.
  • Nordstrom, O.
  • Bartfai, Tamas

publication date

  • 1988

journal

  • Journal of Neuroimmunology  Journal

abstract

  • Rat thymocytes possess a single class of saturable, high affinity binding sites for muscarinic antagonists of the benzilate type such as [3H]3-quinuclidinyl benzilate ([3H]3-QNB). The average number of receptors per cell is 3000 and the equilibrium dissociation constant of [3H]3-QNB on intact cells is 7.5 nM. In the work reported here we found that perturbation of the thymocyte membrane by addition of phytohemagglutinin (4 micrograms/ml) caused a transient increase in muscarinic antagonist binding, and hydrocortisone (100 mg/kg s.c.) treatment of rats for 2 days prior to sacrifice increased the average number of muscarinic receptor sites on thymocytes by 100%. Atropine treatment, which in other tissues causes increased muscarinic receptor concentration, did not alter the receptor number on thymocytes. Binding of carbachol to the receptor on intact cells resulted in inhibition of cAMP synthesis and stimulation of cGMP synthesis. These muscarinic agonist effects were each inhibited by the simultaneous addition of the muscarinic antagonist atropine (5 X 10(-5) M). No stimulation of phosphatidylinositol turnover by muscarinic agonists was observed.

subject areas

  • Animals
  • Male
  • Muscarine
  • Quinuclidines
  • Quinuclidinyl Benzilate
  • Rats
  • Rats, Inbred Strains
  • Receptors, Muscarinic
  • Thymus Gland
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0165-5728

Digital Object Identifier (DOI)

  • 10.1016/0165-5728(88)90118-x

PubMed ID

  • 3339119
scroll to property group menus

Additional Document Info

start page

  • 265

end page

  • 274

volume

  • 17

issue

  • 4

©2019 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support