Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

A unique sorting nexin regulates trafficking of potassium channels via a PDZ domain interaction

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Lunn, M. L.
  • Nassirpour, R.
  • Arrabit, C.
  • Tan, J.
  • McLeod, I.
  • Arias, C. M.
  • Sawchenko, P. E.
  • Yates III, John
  • Slesinger, P. A.

publication date

  • October 2007

journal

  • Nature Neuroscience  Journal

abstract

  • G protein-gated potassium (Kir3) channels are important for controlling neuronal excitability in the brain. Using a proteomics approach, we have identified a unique rodent intracellular protein, sorting nexin 27 (SNX27), which regulates the trafficking of Kir3 channels. Like most sorting nexins, SNX27 possesses a functional PX domain that selectively binds the membrane phospholipid phosphatidylinositol-3-phosphate (PI3P) and is important for trafficking to the early endosome. SNX27, however, is the only sorting nexin to contain a PDZ domain. This PDZ domain discriminates between channels with similar class I PDZ-binding motifs, associating with the C-terminal end of Kir3.3 and Kir3.2c (-ESKV), but not with that of Kir2.1 (-ESEI) or Kv1.4 (-ETDV). SNX27 promotes the endosomal movement of Kir3 channels, leading to reduced surface expression, increased degradation and smaller Kir3 potassium currents. The regulation of endosomal trafficking via sorting nexins reveals a previously unknown mechanism for controlling potassium channel surface expression.

subject areas

  • Animals
  • Brain
  • Cell Line, Transformed
  • Endocytosis
  • G Protein-Coupled Inwardly-Rectifying Potassium Channels
  • Gene Expression Regulation
  • Humans
  • Immunoprecipitation
  • Male
  • Membrane Potentials
  • Molecular Sequence Data
  • Nerve Tissue Proteins
  • PDZ Domains
  • Patch-Clamp Techniques
  • Protein Structure, Tertiary
  • Protein Transport
  • Proteomics
  • Rats
  • Transfection
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 1097-6256

Digital Object Identifier (DOI)

  • 10.1038/nn1953

PubMed ID

  • 17828261
scroll to property group menus

Additional Document Info

start page

  • 1249

end page

  • 1259

volume

  • 10

issue

  • 10

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support