Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Utilization of the methoxymalonyl-acyl carrier protein biosynthesis locus for cloning the oxazolomycin biosynthetic gene cluster from streptomyces albus ja3453

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Zhao, C. H.
  • Ju, J. H.
  • Christenson, S. D.
  • Smith, W. C.
  • Song, D. F.
  • Zhou, X. F.
  • Shen, Ben
  • Deng, Z. X.

publication date

  • June 2006

journal

  • Journal of Bacteriology  Journal

abstract

  • Oxazolomycin (OZM), a hybrid peptide-polyketide antibiotic, exhibits potent antitumor and antiviral activities. Using degenerate primers to clone genes encoding methoxymalonyl-acyl carrier protein (ACP) biosynthesis as probes, a 135-kb DNA region from Streptomyces albus JA3453 was cloned and found to cover the entire OZM biosynthetic gene cluster. The involvement of the cloned genes in OZM biosynthesis was confirmed by deletion of a 12-kb DNA fragment containing six genes for methoxymalonyl-ACP biosynthesis from the specific region of the chromosome, as well as deletion of the ozmC gene within this region, to generate OZM-nonproducing mutants.

subject areas

  • Acyl Carrier Protein
  • Cloning, Molecular
  • DNA, Bacterial
  • Molecular Sequence Data
  • Multigene Family
  • Oxazoles
  • Pyrrolidinones
  • Restriction Mapping
  • Spiro Compounds
  • Streptomyces
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0021-9193

Digital Object Identifier (DOI)

  • 10.1128/jb.00173-06

PubMed ID

  • 16707707
scroll to property group menus

Additional Document Info

start page

  • 4142

end page

  • 4147

volume

  • 188

issue

  • 11

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support