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Mixed-element capture agents: A simple strategy for the construction of synthetic, high-affinity protein capture ligands

Academic Article
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Overview

authors

  • Bachhawat-Sikder, K.
  • Kodadek, Thomas

publication date

  • August 2003

journal

  • Journal of the American Chemical Society  Journal

abstract

  • Demonstration of a simple strategy to generate synthetic high-affinity protein capture agents of practical utility for protein-detecting microarrays. The model study highlights capture of the MBP-Mdm2 fusion protein on a solid support by a linear sequence of peptides that bind to the two individual polypeptide chains.

subject areas

  • Carrier Proteins
  • Kinetics
  • Ligands
  • Maltose-Binding Proteins
  • Nuclear Proteins
  • Protein Binding
  • Proteomics
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-mdm2
  • Recombinant Fusion Proteins
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Identity

International Standard Serial Number (ISSN)

  • 0002-7863

Digital Object Identifier (DOI)

  • 10.1021/ja034912n

PubMed ID

  • 12903996
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Additional Document Info

start page

  • 9550

end page

  • 9551

volume

  • 125

issue

  • 32

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