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Short-term fasting induces profound neuronal autophagy

Academic Article
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Overview

authors

  • Alirezaei, M.
  • Kemball, C. C.
  • Flynn, C. T.
  • Wood, Malcolm R.
  • Whitton, J. Lindsay
  • Kiosses, W. B.

publication date

  • August 2010

journal

  • Autophagy  Journal

abstract

  • Disruption of autophagy--a key homeostatic process in which cytosolic components are degraded and recycled through lysosomes--can cause neurodegeneration in tissue culture and in vivo. Upregulation of this pathway may be neuroprotective, and much effort is being invested in developing drugs that cross the blood brain barrier and increase neuronal autophagy. One well-recognized way of inducing autophagy is by food restriction, which upregulates autophagy in many organs including the liver; but current dogma holds that the brain escapes this effect, perhaps because it is a metabolically privileged site. Here, we have re-evaluated this tenet using a novel approach that allows us to detect, enumerate and characterize autophagosomes in vivo. We first validate the approach by showing that it allows the identification and characterization of autophagosomes in the livers of food-restricted mice. We use the method to identify constitutive autophagosomes in cortical neurons and Purkinje cells, and we show that short-term fasting leads to a dramatic upregulation in neuronal autophagy. The increased neuronal autophagy is revealed by changes in autophagosome abundance and characteristics, and by diminished neuronal mTOR activity in vivo, demonstrated by a reduction in levels of phosphorylated S6 ribosomal protein in Purkinje cells. The increased abundance of autophagosomes in Purkinje cells was confirmed using transmission electron microscopy. Our data lead us to speculate that sporadic fasting might represent a simple, safe and inexpensive means to promote this potentially therapeutic neuronal response.

subject areas

  • Animals
  • Autophagy
  • Caloric Restriction
  • Cerebellum
  • Cerebral Cortex
  • Fasting
  • Liver
  • Mice
  • Mice, Inbred C57BL
  • Microscopy, Confocal
  • Neurons
  • Phagosomes
  • Purkinje Cells
  • Reproducibility of Results
  • TOR Serine-Threonine Kinases
  • Time Factors
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Research

keywords

  • CNS
  • Purkinje cells
  • autophagy
  • confocal microscopy
  • cortex
  • cortical neurons
  • electron microscopy
  • fasting
  • neuroprotection
  • starvation
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Identity

PubMed Central ID

  • PMC3106288

International Standard Serial Number (ISSN)

  • 1554-8627

Digital Object Identifier (DOI)

  • 10.4161/auto.6.6.12376

PubMed ID

  • 20534972
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Additional Document Info

start page

  • 702

end page

  • 710

volume

  • 6

issue

  • 6

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