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Transformation by simian virus 40 of spleen cells from a hyperimmune rabbit: Demonstration of production of specific antibody to the immunizing antigen

Academic Article
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Overview

authors

  • Strosberg, Donny
  • Collins, J. J.
  • Black, P. H.
  • Malamud, D.
  • Wilbert, S.
  • Bloch, K. J.
  • Haber, E.

publication date

  • 1974

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • Extracts of spleen cells obtained from a rabbit hyperimmunized with Type III pneumococcal vaccine and transformed with simian virus 40, were subjected to agarose gel electrophoresis in the presence of an [(125)I]S3 polysaccharide-protein conjugate. Binding of radioactivity in the gamma globulin region was observed. Extracts and media obtained from labeled cell cultures contained a protein that bound to an S3, but not to an S8 or inactivated S3 immunoadsorbent. After elution, the bound protein showed a single band on isoelectric focusing which corresponded in isoelectric point to one of the serum anti-S3 antibodies of the donor rabbit. These observations strongly suggest that a normal committed lymphoid cell may be brought into continuous culture by virus transformation and yet retain its ability to synthesize specific antibody.

subject areas

  • Animals
  • Antibodies
  • Antibodies, Bacterial
  • Antibody Formation
  • Antibody Specificity
  • Antigen-Antibody Reactions
  • Bacterial Vaccines
  • Cell Transformation, Neoplastic
  • Immunization
  • Iodine Radioisotopes
  • Isoelectric Focusing
  • Rabbits
  • Radioimmunoassay
  • Simian virus 40
  • Spleen
  • Streptococcus pneumoniae
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Identity

PubMed Central ID

  • PMC387982

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.71.2.263

PubMed ID

  • 4150021
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Additional Document Info

start page

  • 263

end page

  • 264

volume

  • 71

issue

  • 2

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