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Enzyme-linked-immunosorbent-assay for amitriptyline and other antidepressants using a monoclonal-antibody

Academic Article
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Overview

authors

  • Denis, H.
  • Marullo, S.
  • Hoebeke, J.
  • Strosberg, Donny

publication date

  • September 1986

journal

  • Clinica Chimica Acta  Journal

abstract

  • We describe and evaluate a method to measure amitriptyline and other tricyclic antidepressants by enzyme linked immunosorbent assay, using monoclonal antibody. In this assay, biological samples were first incubated with the antibody; in a second step, free remaining antibody was allowed to bind to lysozyme-nortriptyline coated immunotitration plates. The bound fraction of the monoclonal antibody was revealed with rabbit anti-mouse serum coupled to horseradish peroxidase. The optical density of the reaction product was measured with a colorimeter at 410 nm. Specificity of the antibody was investigated by means of a Farr test showing interferences in therapeutic ranges only for chlorpromazine and phenytoine. Means of intra- and inter-assay variations were 10 and 13%, respectively. The results when compared to those obtained by gas chromatography with a selective nitrogen detector gave a correlation coefficient of 0.897. Finally, the great reliability of the monoclonal antibody, the advantages of a decreased analysis time, low cost and high capacity of the procedure contribute to make this immunoassay most suitable for clinical monitoring and pharmacokinetic studies of tricyclic antidepressants.

subject areas

  • Amitriptyline
  • Antibodies, Monoclonal
  • Antidepressive Agents, Tricyclic
  • Chromatography, Gas
  • Enzyme-Linked Immunosorbent Assay
  • Evaluation Studies as Topic
  • Humans
  • Quality Control
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Identity

International Standard Serial Number (ISSN)

  • 0009-8981

Digital Object Identifier (DOI)

  • 10.1016/0009-8981(86)90059-8

PubMed ID

  • 3769214
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Additional Document Info

start page

  • 257

end page

  • 267

volume

  • 159

issue

  • 3

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