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A method for identification of hiv gp140 binding memory b cells in human blood

Academic Article
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Overview

authors

  • Scheid, J. F.
  • Mouquet, H.
  • Feldhahn, N.
  • Walker, B. D.
  • Pereyra, F.
  • Cutrell, E.
  • Seaman, M. S.
  • Mascola, J. R.
  • Wyatt, Richard
  • Wardemann, H.
  • Nussenzweig, M. C.

publication date

  • 2009

journal

  • Journal of Immunological Methods  Journal

abstract

  • Antibodies to HIV are potentially important reagents for basic and clinical studies. Historically, these reagents have been produced by random cloning of heavy and light chains in phage display libraries [Burton, D.R., Barbas, C.F. III, Persson, M.A.A., Koenig, S., Chanock, R.M., and Lerner, R.A., (1991), A large array of human monoclonal antibodies to type 1 immunodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals. Proc. Natl. Acad. Sci. U. S. A. 88, 10134-10137.] and electrofusion techniques [Buchacher, A., Predl, R., Tauer, C., Purtscher, M., Gruber, G., Heider, R., Steindl, F., Trkola, A., Jungbauer, A., and Katinger, H., (1992), Human monoclonal antibodies against gp41 and gp120 as potential agent for passive immunization. Vaccines 92, 191-195]. Here we describe a method to identify and potentially enrich human memory B cells from HIV infected patients that show serum titers of neutralizing antibodies. When biotinylated gp140 is used to stain peripheral blood mononuclear cells it identifies a distinct population of gp140 binding B cells by flow cytometry.

subject areas

  • Adult
  • B-Lymphocytes
  • Biotinylation
  • Flow Cytometry
  • HIV Antibodies
  • HIV Infections
  • HIV-1
  • Humans
  • Immunologic Memory
  • Male
  • Neutralization Tests
  • env Gene Products, Human Immunodeficiency Virus
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Research

keywords

  • HIV
  • Memory B cells
  • gp140
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Identity

PubMed Central ID

  • PMC2754789

International Standard Serial Number (ISSN)

  • 0022-1759

Digital Object Identifier (DOI)

  • 10.1016/j.jim.2008.11.012

PubMed ID

  • 19100741
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Additional Document Info

start page

  • 65

end page

  • 67

volume

  • 343

issue

  • 2

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