The lifespan-promoting effect of acetic acid and reishi polysaccharide

Academic Article

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Overview

authors

• Chuang, M. H.
• Chiou, S. H.
• Huang, C. H.
• Yang, W. B.
• Wong, Chi-Huey

publication date

• November 2009

journal

• Bioorganic & Medicinal Chemistry  Journal

abstract

• Using Caenorhabditis elegans as a model organism, various natural substances and commercial health-food supplements were screened to evaluate their effects on longevity. Among the substances tested, acetic acid and Reishi polysaccharide fraction 3 (RF3) were shown to increase the expression of the lifespan and longevity-related transcription factor DAF-16 in C. elegans. We have shown that RF3 activates DAF-16 expression via TIR-1 receptor and MAPK pathway whereas acetic acid inhibits the trans-membrane receptor DAF-2 of the insulin/IGF-1 pathway to indirectly activate DAF-16 expression. In addition, a mixture of acetic acid and RF3 possesses a combined effect 30-40% greater than either substance used alone. A proteomic analysis of C. elegans using 2-DE and LC-MS/MS was then carried out, and 15 differentially expressed proteins involved in the lifespan-promoting activity were identified.

subject areas

• Acetic Acid
• Animals
• Caenorhabditis elegans
• Caenorhabditis elegans Proteins
• Chromatography, Liquid
• Electrophoresis, Gel, Two-Dimensional
• Forkhead Transcription Factors
• Gene Expression Regulation
• Insulin-Like Growth Factor I
• Longevity
• Polysaccharides
• Proteomics
• Receptor, Insulin
• Reishi
• Tandem Mass Spectrometry
• Transcription Factors

Research

keywords

• Acetic acid
• DAF-16 forkhead transcription factor
• Insulin/IGF-1 signaling pathway
• Reishi polysaccharide
• Toll-interleukin-1 receptor

Identity

International Standard Serial Number (ISSN)

• 0968-0896

Digital Object Identifier (DOI)

• 10.1016/j.bmc.2009.09.002

PubMed ID

• 19837596

Additional Document Info

start page

• 7831

end page

• 7840

volume

• 17

issue

• 22