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Differences in the degree of depletion, rate of recovery, and the preferential elimination of naive cd4(+) t-cells by anti-cd4 monoclonal-antibody (gk1.5) in young and aged mice

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Overview

authors

  • Rice, James
  • Bucy, R. P.

publication date

  • June 1995

journal

  • Journal of Immunology  Journal

abstract

  • We compared the degrees of depletion and the rates of recovery of CD4+ T cells in young and old mice given a single injection of an anti-CD4 (GK1.5) mAb. We found that in young mice the CD4+ population of T cells was completely depleted from the peripheral blood and, subsequently, the lymph nodes and spleen. Young mice recovered 80% of CD4+ T cells within 100 days after the injection. Although aged mice have less CD4+ T cells, CD4+ T cells were depleted by only 60% after mAb injection. This finding was associated with prolonged circulation of Ab-coated T cells. The rate of repopulation of peripheral CD4+ T cells was also affected by aging, because 5 times less CD4+ T cells appear in aged than in young mice between days 14 and 100 post injection. Further, we found that the CD4+ T cells that are depleted in both young and old mice by injection of anti-CD4 mAb are CD45RBhigh, suggesting a preferential depletion of immature cells. Finally, the thymus appears to be required to repopulate the T cell population post injection, because thymectomized young mice failed to recover CD3+CD4+ cells.

subject areas

  • Aging
  • Animals
  • Antibodies, Monoclonal
  • Antigens, CD3
  • Antigens, CD44
  • Antigens, CD45
  • CD4-Positive T-Lymphocytes
  • Carrier Proteins
  • Cell Differentiation
  • Lymphocyte Count
  • Lymphocyte Depletion
  • Lymphoid Tissue
  • Mice
  • Mice, Inbred BALB C
  • Mice, Inbred C57BL
  • Receptors, Cell Surface
  • Receptors, Lymphocyte Homing
  • Thymectomy
  • Thymus Gland
  • Time Factors
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 7539027
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Additional Document Info

start page

  • 6644

end page

  • 6654

volume

  • 154

issue

  • 12

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