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Robust hepatitis C virus infection in vitro

Academic Article
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Overview

authors

  • Zhong, J.
  • Gastaminza, P.
  • Cheng, G. F.
  • Kapadia, S.
  • Kato, T.
  • Burton, Dennis
  • Wieland, Stefan
  • Uprichard, S. L.
  • Wakita, T.
  • Chisari, Francis

publication date

  • June 2005

journal

  • Proceedings of the National Academy of Sciences of the United States of America  Journal

abstract

  • The absence of a robust cell culture model of hepatitis C virus (HCV) infection has severely limited analysis of the HCV life cycle and the development of effective antivirals and vaccines. Here we report the establishment of a simple yet robust HCV cell culture infection system based on the HCV JFH-1 molecular clone and Huh-7-derived cell lines that allows the production of virus that can be efficiently propagated in tissue culture. This system provides a powerful tool for the analysis of host-virus interactions that should facilitate the discovery of antiviral drugs and vaccines for this important human pathogen.

subject areas

  • Antigens, CD
  • Antigens, CD81
  • Cell Culture Techniques
  • Cell Line
  • Cells, Cultured
  • Centrifugation, Density Gradient
  • Cloning, Molecular
  • DNA, Complementary
  • Fluorescent Antibody Technique, Indirect
  • HL-60 Cells
  • HeLa Cells
  • Hepacivirus
  • Hepatitis C
  • Humans
  • Kinetics
  • Microscopy, Fluorescence
  • Protein Binding
  • RNA
  • Sucrose
  • Time Factors
  • Transcription, Genetic
  • Transfection
  • U937 Cells
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Research

keywords

  • CD81
  • Huh-7
  • interferon
  • viral entry
  • viral spread
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Identity

PubMed Central ID

  • PMC1166622

International Standard Serial Number (ISSN)

  • 0027-8424

Digital Object Identifier (DOI)

  • 10.1073/pnas.0503596102

PubMed ID

  • 15939869
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Additional Document Info

start page

  • 9294

end page

  • 9299

volume

  • 102

issue

  • 26

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