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In vivo regulation of crkii and crkl proto-oncogenes in the uterus by insulin-like growth factor-i - differential effects on tyrosine phosphorylation and association with paxillin

Academic Article
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Overview

authors

  • Butler, Andrew
  • Blakesley, V. A.
  • Koval, A.
  • DeJong, R.
  • Groffen, J.
  • LeRoith, D.

publication date

  • October 1997

journal

  • Journal of Biological Chemistry  Journal

abstract

  • Changes in CrkII and CrkL phosphorylation are associated with insulin-like growth factor receptor activation in cultured cells. We examined whether similar changes also occur following administration of recombinant human insulin-like growth factor-I to the intact animal. In female rats starved overnight, CrkL phosphorylation was significantly increased 12 min after insulin-like growth factor-I administration. Tyrosine phosphorylation of CrkII was not detectable in either control or treated animals. Paxillin, a 65-70-kDa phosphoprotein containing high affinity binding sites common for the Src homology 2 (SH2) domains of CrkII and CrkL, was observed in both CrkII and CrkL immunoprecipitates. Insulin-like growth factor-I treatment stimulated the association of CrkII with paxillin. In contrast, the same treatment resulted in the dissociation of the CrkL-paxillin complex. Similar effects of insulin-like growth factor-I treatment on the association of CrkL with tyrosine phosphorylated paxillin were observed in fibroblasts overexpressing CrkL. This study demonstrates that the activation of the insulin-like growth factor-I receptor induces changes in the tyrosine phosphorylation and protein-protein interactions of the Crk proteins in vivo. The different responses of CrkL and CrkII to insulin-like growth factor-I receptor activation suggest distinct roles for these two adapter proteins in signal transduction.

subject areas

  • 3T3 Cells
  • Adaptor Proteins, Signal Transducing
  • Animals
  • Cytoskeletal Proteins
  • Estrogens
  • Female
  • Gene Expression Regulation, Developmental
  • Insulin Receptor Substrate Proteins
  • Insulin-Like Growth Factor I
  • Mice
  • Nuclear Proteins
  • Ovariectomy
  • Paxillin
  • Phosphoproteins
  • Phosphorylation
  • Protein Binding
  • Protein Kinases
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-crk
  • Rats
  • Rats, Sprague-Dawley
  • Receptor, IGF Type 1
  • Tyrosine
  • Uterus
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

Digital Object Identifier (DOI)

  • 10.1074/jbc.272.44.27660

PubMed ID

  • 9346905
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Additional Document Info

start page

  • 27660

end page

  • 27664

volume

  • 272

issue

  • 44

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