Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form

Expression of an animal virus antigenic site on the surface of a plant-virus particle

Academic Article
uri icon
  • Overview
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Usha, R.
  • Rohll, J. B.
  • Spall, V. E.
  • Shanks, M.
  • Maule, A. J.
  • Johnson Jr., John
  • Lomonossoff, G. P.

publication date

  • November 1993

journal

  • Virology  Journal

abstract

  • To investigate if cowpea mosaic virus (CPMV) particles can be used to express foreign protein sequences, oligonucleotides encoding an epitope derived from VP1 of foot-and-mouth disease virus (FMDV) were cloned into the region of the CPMV genome encoding the small (S) coat protein. The chimeras were designed so that the foreign sequence was expressed either as an insertion or as a replacement for part of the wild-type sequence. While RNA from both chimeras was able to replicate in cowpea protoplasts only the construct containing the FMDV sequence as an insertion was able to direct capsid formation and infect whole cowpea plants. The modified S protein produced in plants infected with the insertion derivative reacted with FMDV-specific antiserum. These results show that CPMV can be used as an antigen presentation system and raises the possibility of producing vaccines in plants using a RNA virus-based vector.

subject areas

  • Amino Acid Sequence
  • Antigens, Viral
  • Aphthovirus
  • Base Sequence
  • Blotting, Northern
  • Capsid
  • Comovirus
  • Epitopes
  • Gene Expression
  • Genome, Viral
  • Microscopy, Immunoelectron
  • Models, Structural
  • Molecular Sequence Data
  • Mutagenesis, Insertional
  • Plants
  • Plasmids
  • Protein Structure, Secondary
  • Protoplasts
  • RNA, Viral
  • Recombinant Fusion Proteins
  • Restriction Mapping
  • Transcription, Genetic
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0042-6822

Digital Object Identifier (DOI)

  • 10.1006/viro.1993.1598

PubMed ID

  • 7692669
scroll to property group menus

Additional Document Info

start page

  • 366

end page

  • 374

volume

  • 197

issue

  • 1

©2021 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support