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Plasminogen interacts with human platelets through two distinct mechanisms

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Overview

authors

  • Miles, Lindsey
  • Ginsberg, Mark
  • White, J. G.
  • Plow, E. F.

publication date

  • June 1986

journal

  • Journal of Clinical Investigation  Journal

abstract

  • Glu-plasminogen, the native form of plasminogen, interacts in a specific and saturable manner with unstimulated human platelets, and the binding is enhanced fivefold by thrombin stimulation (Miles and Plow, 1985. J. Biol. Chem. 260:4303). This study characterizes the nature of the Glu-plasminogen binding sites by analyzing platelets deficient in selected proteins and functions. Platelets from patients with afibrinogenemia, Gray platelet syndrome, and the Cam Variant of thrombasthenia, a form of thrombasthenia with near normal levels of glycoprotein IIb/IIIa (GPIIb/IIIa), showed minimal augmentation of plasminogen binding to thrombin-stimulated platelets but normal binding to unstimulated platelets. This selective deficiency indicates that two distinct mechanisms are involved in the interaction of plasminogen with platelets. These abnormal platelets share a deficiency in fibrinogen. Surface expression of platelet fibrinogen, however, was not sufficient for enhanced plasminogen binding to stimulated platelets, and experiments with alpha-thrombin and gamma-thrombin indicated that fibrin formation on the platelet surface is necessary for the augmented plasminogen binding. Unstimulated and stimulated thrombasthenic platelets deficient in GPIIb/IIIa bound markedly reduced levels of plasminogen, which suggests a role for GPIIb/IIIa in plasminogen binding to unstimulated platelets. Treatment of platelets to dissociate the heterodimeric complex of GPIIb/IIIa did not significantly perturb plasminogen binding to unstimulated platelets, but the complex may be necessary for thrombin-stimulated plasminogen binding via its interaction with platelet fibrin.

subject areas

  • Blood Platelets
  • Calcimycin
  • Epinephrine
  • Fibrinogen
  • Fibrinopeptide A
  • Humans
  • Immunoglobulin Fab Fragments
  • Molecular Weight
  • Plasminogen
  • Thrombin
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Identity

PubMed Central ID

  • PMC370561

International Standard Serial Number (ISSN)

  • 0021-9738

Digital Object Identifier (DOI)

  • 10.1172/jci112529

PubMed ID

  • 3086385
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Additional Document Info

start page

  • 2001

end page

  • 2009

volume

  • 77

issue

  • 6

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