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Pulmonary surfactant proteins a and d directly suppress cd3(+)/cd4(+) cell function: Evidence for two shared mechanisms(1)

Academic Article
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Overview

authors

  • Borron, P. J.
  • Mostaghel, E. A.
  • Doyle, C.
  • Walsh, E. S.
  • McHeyzer-Williams, Michael G.
  • Wright, J. R.

publication date

  • November 2002

journal

  • Journal of Immunology  Journal

abstract

  • Pulmonary surfactant is a lipoprotein complex that lowers surface tension at the air-liquid interface of the lung and participates in pulmonary host defense. Surfactant proteins (SP), SP-A and SP-D, modulate a variety of immune cell functions, including the production of cytokines and free radicals. Previous studies showed that SP-A and SP-D inhibit lymphocyte proliferation in the presence of accessory cells. The goal of this study was to determine whether SP-A and SP-D directly suppress Th cell function. Both proteins inhibited CD3(+)/CD4(+) lymphocyte proliferation induced by PMA and ionomycin in an IL-2-independent manner. Both proteins decreased the number of cells entering the S and mitotic phases of the cell cycle. Neither SP-A nor SP-D altered cell viability, apoptosis, or secretion of IL-2, IL-4, or IFN-gamma when Th cells were treated with PMA and ionomycin. However, both proteins attenuated ionomycin-induced cytosolic free calcium ([Ca(2+) ](i)), but not thapsigargin-induced changes in [Ca(2+)](i). In summary, inhibition of T cell proliferation by SP-A and SP-D occurs via two mechanisms, an IL-2-dependent mechanism observed with accessory cell-dependent T cell mitogens and specific Ag, as well as an IL-2-independent mechanism of suppression that potentially involves attenuation of [Ca(2+)](i).

subject areas

  • Amino Acid Sequence
  • Animals
  • Antigens, CD3
  • CD4-Positive T-Lymphocytes
  • Calcium
  • Cattle
  • Cell Cycle
  • Coculture Techniques
  • Cytosol
  • Down-Regulation
  • Epitopes, T-Lymphocyte
  • Growth Inhibitors
  • Humans
  • Hybridomas
  • Immunosuppressive Agents
  • Interleukin-2
  • Ionomycin
  • Leukocytes, Mononuclear
  • Lymphocyte Activation
  • Mice
  • Mice, Inbred C57BL
  • Molecular Sequence Data
  • Pulmonary Surfactant-Associated Protein A
  • Pulmonary Surfactant-Associated Protein D
  • Rats
  • T-Lymphocyte Subsets
  • T-Lymphocytes, Helper-Inducer
  • Tetradecanoylphorbol Acetate
  • Thapsigargin
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Identity

International Standard Serial Number (ISSN)

  • 0022-1767

PubMed ID

  • 12421966
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Additional Document Info

start page

  • 5844

end page

  • 5850

volume

  • 169

issue

  • 10

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