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Monoclonal antibodies to cytochrome p-450 immunopurify a 45-kda protein from a human lymphoblastoid cell line

Academic Article
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Overview

authors

  • Friedman, F. K.
  • Pastewka, J. V.
  • Robinson, R. C.
  • Park, S. S.
  • Marletta, Michael
  • Gelboin, H. V.

publication date

  • 1985

journal

  • FEBS Letters  Journal

abstract

  • Monoclonal antibodies (MAbs) to rat liver cytochromes P-450 have previously been used for successful immunopurification of cytochromes P-450 from animal tissues. We now report application of this MAb-based immunopurification technique to the human lymphoblastoid AHH-1 cell line. Immunopurification carried out with 3 different MAbs each yielded a 45-kDa polypeptide. The purified protein contains an MAb-specific epitope present on cytochromes P-450, and may therefore be a human cytochrome P-450.

subject areas

  • Animals
  • Antibodies, Monoclonal
  • Antibody Specificity
  • Cell Line
  • Cytochrome P-450 Enzyme System
  • Enzyme Induction
  • Epitopes
  • Humans
  • Immunologic Techniques
  • Liver
  • Lymphocytes
  • Methylcholanthrene
  • Phenobarbital
  • Rats
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Identity

International Standard Serial Number (ISSN)

  • 0014-5793

Digital Object Identifier (DOI)

  • 10.1016/0014-5793(85)80742-0

PubMed ID

  • 2581816
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Additional Document Info

start page

  • 67

end page

  • 70

volume

  • 185

issue

  • 1

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