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Carbohydrate on human factor viii von willebrand factor - impairment of function by removal of specific galactose residues

Academic Article
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Overview

authors

  • Sodetz, J. M.
  • Paulson, James
  • Pizzo, S. V.
  • McKee, P. A.

publication date

  • 1978

journal

  • Journal of Biological Chemistry  Journal

abstract

  • Human factor VIII/von Willebrand factor protein containing 120 +/- 12 nmol of sialic acid and 135 +/- 13 nmol of galactose/mg of protein was digested with neuraminidase. The affinity of native factor VIII/von Willebrand factor and its asialo form for the hepatic lectin that specifically binds asialoglycoproteins was assessed from in vitro binding experiments. Native factor VIII/von Willebrand factor exhibited negligible affinity while binding of the asialo derivative was comparable to that observed for asialo-alpha1-acid glycoprotein. Incubation of asialo-factor VIII/von Willebrand factor with Streptococcus pneumoniae beta-galactosidase removed only 62% of the galactose but abolished binding to the purified hepatic lectin. When the asialo derivative was incubated with purified beta-D-galactoside alpha2 leads to 6 sialyltransferase and CMP-[14C]NeuAc, only 61% of the galactose incorporated [14C]NeuAc. From the known specificites of these enzymes, it is concluded that galactose residues important in lectin binding are present in a terminal Gal/beta1 leads to 4GlcNAc sequence on asialo-factor VIII/von Willebrand factor. The relative ristocetin-induced platelet aggregating activity of native, asialo-, and agalacto-factor VIII/von Willebrand factor was 100:38:12, respectively, while procoagulant activity was 100:100:103.

subject areas

  • Animals
  • Blood Coagulation Factors
  • Factor VIII
  • Galactose
  • Humans
  • Immunodiffusion
  • Lectins
  • Liver
  • Neuraminidase
  • Platelet Aggregation
  • Rabbits
  • Sialic Acids
  • beta-Galactosidase
  • von Willebrand Factor
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Identity

International Standard Serial Number (ISSN)

  • 0021-9258

PubMed ID

  • 100492
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Additional Document Info

start page

  • 7202

end page

  • 7206

volume

  • 253

issue

  • 20

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