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Intrathecal administration of PNA targeting galanin receptor reduces galanin-mediated inhibitory effect in the rat spinal cord

Academic Article
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Overview

authors

  • Rezaei, K.
  • Xu, I. S.
  • Wu, W. P.
  • Shi, T. J.
  • Soomets, U.
  • Land, T.
  • Xu, X. J.
  • Wiesenfeld-Hallin, Z.
  • Hokfelt, T.
  • Bartfai, Tamas
  • Langel, Ülo

publication date

  • February 2001

journal

  • Neuroreport  Journal

abstract

  • Peptide nucleic acids (PNA) are nucleic acid analogues containing neutral amide backbone, forming stable and tight complexes with complementary DNA/RNA. However, it is unclear whether unmodified PNA can efficiently penetrate neuronal tissue in order to act as antisense reagent. Here we show that intrathecal (i.t.) injection of an unmodified antisense PNA complementary to the rat galanin receptor type 1 (GalR1) mRNA is able to block the inhibitory effect of i.t. administered galanin on spinal nociceptive transmission. Autoradiographic ligand binding studies using [125I]galanin show that the unmodified PNA is able to reduce the density of galanin binding sites in the dorsal horn. Thus, unmodified PNA applied i.t. appears to function as an effective antisense reagent in rat spinal cord in vivo.

subject areas

  • Animals
  • Autoradiography
  • Drug Delivery Systems
  • Electrophysiology
  • Female
  • Galanin
  • Injections, Spinal
  • Iodine Radioisotopes
  • Ligands
  • Nerve Fibers
  • Neurons, Afferent
  • Nociceptors
  • Oligonucleotides, Antisense
  • Peptide Nucleic Acids
  • Rats
  • Rats, Sprague-Dawley
  • Receptor, Galanin, Type 1
  • Receptors, Galanin
  • Receptors, Neuropeptide
  • Spinal Cord
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Research

keywords

  • antisense
  • drug delivery
  • neuropeptides
  • pain
  • sensory neurons
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Identity

International Standard Serial Number (ISSN)

  • 0959-4965

Digital Object Identifier (DOI)

  • 10.1097/00001756-200102120-00027

PubMed ID

  • 11209942
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Additional Document Info

start page

  • 317

end page

  • 320

volume

  • 12

issue

  • 2

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