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Application of a multiprobe RNase protection assay and junctional sequences to define V beta gene diversity in Sezary-syndrome

Academic Article
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Overview

authors

  • Kono, Dwight
  • BaccalĂ , Roberto
  • Balderas, R. S.
  • Kovac, S. J.
  • Heald, P. W.
  • Edelson, R. L.
  • Theofilopoulos, Argyrios

publication date

  • April 1992

journal

  • American Journal of Pathology  Journal

abstract

  • Nineteen patients with mycosis fungoides/Sezary syndrome (MF/SZ), a malignancy of the mature helper T-cell phenotype (CD4+TCR alpha beta+), were screened for clonotypic V beta expansions in peripheral blood with a multiprobe RNase protection assay. A different predominant V beta gene was identified in 9 of 14 patients with high peripheral blood CD4/CD8 ratios, whereas 4 of these patients showed T-cell expansions expressing V beta genes other than those included in the assay. In contrast, five patients with few, if any, malignant cells in the circulation had V beta expression levels similar to that in normal peripheral blood. A unique V-D-J sequence was found for each highly expressed V beta gene, thereby documenting monoclonality of the expanded T-cell populations. Polymerase chain reaction (PCR) primers specific for the D-J beta junction accurately identified the corresponding malignant clonotype in peripheral blood. The diverse TCR V beta gene usage found in these MF/SZ patients suggests that T-cell receptor (TCR) specificity has no bearing on this disease.

subject areas

  • Adult
  • Aged
  • Aged, 80 and over
  • Base Sequence
  • Cloning, Molecular
  • Female
  • Gene Expression Regulation, Neoplastic
  • Genetic Techniques
  • Genetic Variation
  • Humans
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Mycosis Fungoides
  • Oligonucleotide Probes
  • Polymerase Chain Reaction
  • Receptors, Antigen, T-Cell
  • Ribonucleases
  • Sezary Syndrome
  • Skin Neoplasms
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Identity

PubMed Central ID

  • PMC1886373

International Standard Serial Number (ISSN)

  • 0002-9440

PubMed ID

  • 1562047
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Additional Document Info

start page

  • 823

end page

  • 830

volume

  • 140

issue

  • 4

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