Scripps VIVO scripps research logo

  • Index
  • Log in
  • Home
  • People
  • Organizations
  • Research
  • Events
Search form
As of April 1st VIVO Scientific Profiles will no longer updated for faculty, and the link to VIVO will be removed from the library website. Faculty profile pages will continue to be updated via Interfolio. VIVO will continue being used behind the scenes to update graduate student profiles. Please contact helplib@scripps.edu if you have questions.
How to download citations from VIVO | Alternative profile options

Neurotrophin-3- and norepinephrine-mediated adrenergic differentiation and the inhibitory action of desipramine and cocaine

Academic Article
uri icon
  • Overview
  • Research
  • Identity
  • Additional Document Info
  • View All
scroll to property group menus

Overview

authors

  • Zhang, J. M.
  • Dix, J.
  • LangtimmSedlak, C. J.
  • Trusk, T.
  • Schroeder, B.
  • Hoffmann, R.
  • Strosberg, Donny
  • Winslow, J. W.
  • SieberBlum, M.

publication date

  • March 1997

journal

  • Journal of Neurobiology  Journal

abstract

  • In the presence of neurotrophin-3 (NT-3), high-affinity norepinephrine (NE) uptake by quail neural crest cells was significantly increased as judged by in vitro colony assay of adrenergic differentiation. In the presence of the related neurotrophins nerve growth factor (NGF) or brain-derived neurotrophic (BDNF) factor, or of basic fibroblast growth factor (bFGF), there were no significant changes. When NE was added to the culture medium in addition to NT-3, more colonies contained dopamine-beta-hydroxylase (DBH)-immunoreactive cells, an enzyme that is characteristic for adrenergic cells. The NE-mediated increase in the portion of colonies that contained DBH-immunoreactive cells was prevented by the tricyclic antidepressant desipramine (DMI) and by cocaine, two types of drug that block cellular transport of NE. To further examine whether NE acts via uptake, colony assays were performed in the presence and absence of adrenergic antagonists and agonists. These would be expected to mimic the DMI and NE effects, respectively, if the mechanism of action involved activation of adrenergic autoreceptors. Neither class of drug showed a detectable effect within a wide range of concentrations. Immunocytochemistry using antibodies against beta 1 and beta 2 adrenergic receptors further supported the notion that DMI action and beta-receptor expression are not causally related. Ratio imaging was subsequently used in an attempt to elucidate the mechanism of NE action. Within a few minutes of addition of NE to the culture medium, there was an increase in intracellular free calcium in a subset of neural crest cells. Taken together, our data indicate that NT-3 is involved in the appearance of the NE transporter (NET) during embryonic development; internalized NE directly or indirectly increases adrenergic differentiation as measured by immunoreactivity of the adrenergic biosynthetic enzyme DBH; and norepinephrine uptake inhibitors have treatogenic potential.

subject areas

  • Adrenergic Uptake Inhibitors
  • Adrenergic beta-Agonists
  • Animals
  • Cell Differentiation
  • Cocaine
  • Desipramine
  • Dopamine beta-Hydroxylase
  • Nerve Growth Factors
  • Neural Crest
  • Neurons
  • Neurotrophin 3
  • Norepinephrine
  • Quail
  • Receptors, Adrenergic
  • Sympathetic Nervous System
scroll to property group menus

Research

keywords

  • cocaine
  • desipramine
  • dopamine-beta-hydroxylase
  • neural crest
  • neurotrophin-3
  • norepinephrine uptake
scroll to property group menus

Identity

International Standard Serial Number (ISSN)

  • 0022-3034

Digital Object Identifier (DOI)

  • 10.1002/(sici)1097-4695(199703)32:3<262::aid-neu2>3.0.co;2-5

PubMed ID

  • 9058320
scroll to property group menus

Additional Document Info

start page

  • 262

end page

  • 280

volume

  • 32

issue

  • 3

©2022 The Scripps Research Institute | Terms of Use | Powered by VIVO

  • About
  • Contact Us
  • Support